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Cloning and expression of a novel, truncated, progesterone receptor.

Publication ,  Journal Article
Saner, KJ; Welter, BH; Zhang, F; Hansen, E; Dupont, B; Wei, Y; Price, TM
Published in: Mol Cell Endocrinol
February 28, 2003

Progesterone acts via two specific receptors to affect gene transcription in target tissues. Progesterone receptor (PR) B contains 933 amino acids while PR A is a truncated version lacking the initial 164 amino acids. We have cloned a novel, truncated PR from both human adipose and aortic cDNA libraries. This cDNA encodes a predicted protein of 314 amino acids, termed PR-M. Initiation of transcription of PR-M occurs in intron 3, with the initial exon identical to exon 4 of the genomic PRs, except for a novel 16 amino acid amino-terminal sequence, consistent with a signal peptide. The remainder of PR-M is identical to the genomic PR. Transcript for this protein was identified by RT-PCR in human aortic endothelial cells and T47D breast cancer cells. Expression of PR-M in Sf 9 insect cells results in a 38-kDa protein, demonstrated in human aortic endothelial cells and T47D breast cancer cells. The function of PR-M remains to be determined. The presence of a signal peptide and the lack of a DNA binding region suggests a non-genomic action.

Duke Scholars

Published In

Mol Cell Endocrinol

DOI

ISSN

0303-7207

Publication Date

February 28, 2003

Volume

200

Issue

1-2

Start / End Page

155 / 163

Location

Ireland

Related Subject Headings

  • Receptors, Progesterone
  • Protein Structure, Tertiary
  • Protein Sorting Signals
  • Molecular Sequence Data
  • Humans
  • Gene Library
  • Female
  • Endothelial Cells
  • Endocrinology & Metabolism
  • Cloning, Molecular
 

Citation

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Saner, K. J., Welter, B. H., Zhang, F., Hansen, E., Dupont, B., Wei, Y., & Price, T. M. (2003). Cloning and expression of a novel, truncated, progesterone receptor. Mol Cell Endocrinol, 200(1–2), 155–163. https://doi.org/10.1016/s0303-7207(02)00380-5
Saner, Karla J., Brenda H. Welter, Fan Zhang, Elizabeth Hansen, Barbara Dupont, Yangzhan Wei, and Thomas M. Price. “Cloning and expression of a novel, truncated, progesterone receptor.Mol Cell Endocrinol 200, no. 1–2 (February 28, 2003): 155–63. https://doi.org/10.1016/s0303-7207(02)00380-5.
Saner KJ, Welter BH, Zhang F, Hansen E, Dupont B, Wei Y, et al. Cloning and expression of a novel, truncated, progesterone receptor. Mol Cell Endocrinol. 2003 Feb 28;200(1–2):155–63.
Saner, Karla J., et al. “Cloning and expression of a novel, truncated, progesterone receptor.Mol Cell Endocrinol, vol. 200, no. 1–2, Feb. 2003, pp. 155–63. Pubmed, doi:10.1016/s0303-7207(02)00380-5.
Saner KJ, Welter BH, Zhang F, Hansen E, Dupont B, Wei Y, Price TM. Cloning and expression of a novel, truncated, progesterone receptor. Mol Cell Endocrinol. 2003 Feb 28;200(1–2):155–163.
Journal cover image

Published In

Mol Cell Endocrinol

DOI

ISSN

0303-7207

Publication Date

February 28, 2003

Volume

200

Issue

1-2

Start / End Page

155 / 163

Location

Ireland

Related Subject Headings

  • Receptors, Progesterone
  • Protein Structure, Tertiary
  • Protein Sorting Signals
  • Molecular Sequence Data
  • Humans
  • Gene Library
  • Female
  • Endothelial Cells
  • Endocrinology & Metabolism
  • Cloning, Molecular