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MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response.

Publication ,  Journal Article
Kilgore, MW; Tate, PL; Rai, S; Sengoku, E; Price, TM
Published in: Mol Cell Endocrinol
May 16, 1997

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear receptors that regulate transcription of target genes. Since attempts have been made to correlate the ingestion of high-fat diets, itself a peroxisome proliferator, with the occurrence of breast cancer, we set about to determine if human breast cancer cells contained a functional PPAR. In this report we demonstrate the presence of an mRNA in two breast cancer cell lines (MCF-7 and T47D) which is specifically recognized by a mouse PPARgamma2 probe. Furthermore, in gel shift assays a consensus PPAR response element (PPRE) was specifically bound by nuclear extracts from MCF-7 cells and was further retarded by antibodies raised to mouse PPARgamma. Finally, when transfected with a PPRE-luciferase transcriptional reporter construct, transcription was increased in response to activators of PPAR and its dimmeric partner the retinoic acid X receptor (RXR). These data indicate that peroxisomal proliferators are capable of mediating transcription in human breast cells and suggest the possibility of a physiological role in the breast.

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Published In

Mol Cell Endocrinol

DOI

ISSN

0303-7207

Publication Date

May 16, 1997

Volume

129

Issue

2

Start / End Page

229 / 235

Location

Ireland

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Receptors, Cytoplasmic and Nuclear
  • RNA, Messenger
  • Humans
  • Gene Expression Regulation
  • Endocrinology & Metabolism
  • Breast Neoplasms
  • Blotting, Northern
 

Citation

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Kilgore, M. W., Tate, P. L., Rai, S., Sengoku, E., & Price, T. M. (1997). MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response. Mol Cell Endocrinol, 129(2), 229–235. https://doi.org/10.1016/s0303-7207(97)04057-4
Kilgore, M. W., P. L. Tate, S. Rai, E. Sengoku, and T. M. Price. “MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response.Mol Cell Endocrinol 129, no. 2 (May 16, 1997): 229–35. https://doi.org/10.1016/s0303-7207(97)04057-4.
Kilgore MW, Tate PL, Rai S, Sengoku E, Price TM. MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response. Mol Cell Endocrinol. 1997 May 16;129(2):229–35.
Kilgore, M. W., et al. “MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response.Mol Cell Endocrinol, vol. 129, no. 2, May 1997, pp. 229–35. Pubmed, doi:10.1016/s0303-7207(97)04057-4.
Kilgore MW, Tate PL, Rai S, Sengoku E, Price TM. MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response. Mol Cell Endocrinol. 1997 May 16;129(2):229–235.
Journal cover image

Published In

Mol Cell Endocrinol

DOI

ISSN

0303-7207

Publication Date

May 16, 1997

Volume

129

Issue

2

Start / End Page

229 / 235

Location

Ireland

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Receptors, Cytoplasmic and Nuclear
  • RNA, Messenger
  • Humans
  • Gene Expression Regulation
  • Endocrinology & Metabolism
  • Breast Neoplasms
  • Blotting, Northern