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Anatomical and physiological localization of prelabeled grafts in rat hippocampus.

Publication ,  Journal Article
Pyapali, GK; Turner, DA; Madison, RD
Published in: Exp Neurol
May 1992

Dissociated rat fetal hippocampal cells were grafted into normal adult rats. The fetal cells were incubated with one of a number of fluorescent compounds at the time of the dissociation to facilitate identification of the individual grafted cells. The fluorescent labels which were analyzed for this purpose included rhodamine latex microspheres, Cascade blue latex beads, rhodamine-dextran-amine, DiI, and carboxyfluorescein ester. The labeled cells were stereotaxically placed as a suspension into normal rat host hippocampi. The rats were sacrificed 2 to 6 weeks after the grafting for in vitro physiological recordings, and the prelabeled grafts were located using fluorescence optics. During intracellular recordings neurons within the prelabeled grafts were injected with Lucifer yellow to visualize the morphology and integration of neuronal processes within the host. Following the recordings the host slices with the grafts were fixed in 4% paraformaldehyde for anatomical analysis. The ability to prelabel cellular grafts allows subsequent anatomical and physiological analysis of the integration of grafted neurons at the resolution of a single neuron. Such an analysis will improve our understanding of the survival, differentiation, migration, and integration of the grafted neurons and their potential to replace lost function in the lesioned hippocampus.

Duke Scholars

Published In

Exp Neurol

DOI

ISSN

0014-4886

Publication Date

May 1992

Volume

116

Issue

2

Start / End Page

133 / 144

Location

United States

Related Subject Headings

  • Rats
  • Neurology & Neurosurgery
  • Microspheres
  • Hippocampus
  • Fluorescent Dyes
  • Fetal Tissue Transplantation
  • Evaluation Studies as Topic
  • Animals
  • 5202 Biological psychology
  • 3209 Neurosciences
 

Citation

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Pyapali, G. K., Turner, D. A., & Madison, R. D. (1992). Anatomical and physiological localization of prelabeled grafts in rat hippocampus. Exp Neurol, 116(2), 133–144. https://doi.org/10.1016/0014-4886(92)90161-i
Pyapali, G. K., D. A. Turner, and R. D. Madison. “Anatomical and physiological localization of prelabeled grafts in rat hippocampus.Exp Neurol 116, no. 2 (May 1992): 133–44. https://doi.org/10.1016/0014-4886(92)90161-i.
Pyapali GK, Turner DA, Madison RD. Anatomical and physiological localization of prelabeled grafts in rat hippocampus. Exp Neurol. 1992 May;116(2):133–44.
Pyapali, G. K., et al. “Anatomical and physiological localization of prelabeled grafts in rat hippocampus.Exp Neurol, vol. 116, no. 2, May 1992, pp. 133–44. Pubmed, doi:10.1016/0014-4886(92)90161-i.
Pyapali GK, Turner DA, Madison RD. Anatomical and physiological localization of prelabeled grafts in rat hippocampus. Exp Neurol. 1992 May;116(2):133–144.
Journal cover image

Published In

Exp Neurol

DOI

ISSN

0014-4886

Publication Date

May 1992

Volume

116

Issue

2

Start / End Page

133 / 144

Location

United States

Related Subject Headings

  • Rats
  • Neurology & Neurosurgery
  • Microspheres
  • Hippocampus
  • Fluorescent Dyes
  • Fetal Tissue Transplantation
  • Evaluation Studies as Topic
  • Animals
  • 5202 Biological psychology
  • 3209 Neurosciences