Induction of neuronal morphology in adrenal chromaffin cells cocultured with denervated Schwann cells.

Journal Article (Journal Article)

We have studied the interactions of adrenal chromaffin and Schwann cells in a coculture system to observe whether denervated Schwann cells induce and support chromaffin cell differentiation in a manner analogous to nerve growth factor (NGF). Schwann cells induce both the accumulation of intense clumps of cocultured chromaffin cells on their surfaces and intense neurite outgrowth. This interaction is not blocked by antibodies to NGF or laminin. Conditioned medium from Schwann cell cultures fosters neurite outgrowth in chromaffin cells in a fashion qualitatively similar to NGF. Our data indicate that denervated Schwann cells exert a profound aggregating and differentiating effect upon chromaffin cells, inducing the expression of a neuronal phenotype via a predominantly NGF-independent mechanism.

Full Text

Duke Authors

Cited Authors

  • Paramore, CG; Turner, DA; Madison, RD

Published Date

  • June 1993

Published In

Volume / Issue

  • 121 / 2

Start / End Page

  • 288 - 294

PubMed ID

  • 8339779

International Standard Serial Number (ISSN)

  • 0014-4886

Digital Object Identifier (DOI)

  • 10.1006/exnr.1993.1099


  • eng

Conference Location

  • United States