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Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans.

Publication ,  Journal Article
Liedtke, W; Tobin, DM; Bargmann, CI; Friedman, JM
Published in: Proc Natl Acad Sci U S A
November 25, 2003

All animals detect osmotic and mechanical stimuli, but the molecular basis for these responses is incompletely understood. The vertebrate transient receptor potential channel vanilloid subfamily 4 (TRPV4) (VR-OAC) cation channel has been suggested to be an osmo/mechanosensory channel. To assess its function in vivo, we expressed TRPV4 in Caenorhabditis elegans sensory neurons and examined its ability to generate behavioral responses to sensory stimuli. C. elegans ASH neurons function as polymodal sensory neurons that generate a characteristic escape behavior in response to mechanical, osmotic, or olfactory stimuli. These behaviors require the TRPV channel OSM-9 because osm-9 mutants do not avoid nose touch, high osmolarity, or noxious odors. Expression of mammalian TRPV4 in ASH neurons of osm-9 worms restored avoidance responses to hypertonicity and nose touch, but not the response to odorant repellents. Mutations known to reduce TRPV4 channel activity also reduced its ability to direct nematode avoidance behavior. TRPV4 function in ASH required the endogenous C. elegans osmotic and nose touch avoidance genes ocr-2, odr-3, osm-10, and glr-1, indicating that TRPV4 is integrated into the normal ASH sensory apparatus. The osmotic and mechanical avoidance responses of TRPV4-expressing animals were different in their sensitivity and temperature dependence from the responses of wild-type animals, suggesting that the TRPV4 channel confers its characteristic properties on the transgenic animals' behavior. These results provide evidence that TRPV4 can function as a component of an osmotic/mechanical sensor in vivo.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 25, 2003

Volume

100 Suppl 2

Issue

Suppl 2

Start / End Page

14531 / 14536

Location

United States

Related Subject Headings

  • Transfection
  • TRPV Cation Channels
  • Recombinant Proteins
  • Rats
  • Promoter Regions, Genetic
  • Neurons, Afferent
  • Mammals
  • Ion Channels
  • Cricetinae
  • Cation Transport Proteins
 

Citation

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MLA
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Liedtke, W., Tobin, D. M., Bargmann, C. I., & Friedman, J. M. (2003). Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans. Proc Natl Acad Sci U S A, 100 Suppl 2(Suppl 2), 14531–14536. https://doi.org/10.1073/pnas.2235619100
Liedtke, Wolfgang, David M. Tobin, Cornelia I. Bargmann, and Jeffrey M. Friedman. “Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans.Proc Natl Acad Sci U S A 100 Suppl 2, no. Suppl 2 (November 25, 2003): 14531–36. https://doi.org/10.1073/pnas.2235619100.
Liedtke W, Tobin DM, Bargmann CI, Friedman JM. Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2003 Nov 25;100 Suppl 2(Suppl 2):14531–6.
Liedtke, Wolfgang, et al. “Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans.Proc Natl Acad Sci U S A, vol. 100 Suppl 2, no. Suppl 2, Nov. 2003, pp. 14531–36. Pubmed, doi:10.1073/pnas.2235619100.
Liedtke W, Tobin DM, Bargmann CI, Friedman JM. Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2003 Nov 25;100 Suppl 2(Suppl 2):14531–14536.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 25, 2003

Volume

100 Suppl 2

Issue

Suppl 2

Start / End Page

14531 / 14536

Location

United States

Related Subject Headings

  • Transfection
  • TRPV Cation Channels
  • Recombinant Proteins
  • Rats
  • Promoter Regions, Genetic
  • Neurons, Afferent
  • Mammals
  • Ion Channels
  • Cricetinae
  • Cation Transport Proteins