Skip to main content

Neutrophil depletion attenuates interleukin-8 production in mild-overstretch ventilated normal rabbit lung.

Publication ,  Journal Article
Kotani, M; Kotani, T; Ishizaka, A; Fujishima, S; Koh, H; Tasaka, S; Sawafuji, M; Ikeda, E; Moriyama, K; Kotake, Y; Morisaki, H; Aikawa, N ...
Published in: Crit Care Med
February 2004

OBJECTIVE: Acute lung injury induced by lung overstretch is associated with neutrophil influx, but the pathogenic role of neutrophils in overstretch-induced lung injury remains unclear. DESIGN: To assess the contribution of neutrophils, we compared the effects of noninjurious large tidal volume (Vt) ventilation on lungs in normal and neutrophil-depleted animals. SETTING: Research animal laboratory. SUBJECTS: Twenty-six male Japanese white rabbits. INTERVENTIONS: Animals were mechanically ventilated for 4 hrs with one of the three following protocols: large Vt (20 mL/kg), small Vt (8 mL/kg), and large Vt (20 mL/kg) with neutrophil depletion achieved by a single dose of vinblastine injection (0.75 mg/kg) intravenously 4 days before the experiment. MEASUREMENTS AND MAIN RESULTS: Large Vt ventilation produced alveolar neutrophil influx compared with low Vt (p =.002) without evidence of edema or increased epithelial permeability. The neutrophil influx was accompanied by increases in interleukin-8 in bronchoalveolar lavage fluid (p =.04). Immunohistochemistry of large Vt lungs showed increased interleukin-8 staining in bronchial epithelial cells, alveolar epithelium, alveolar macrophages, and smooth muscles of pulmonary vessels. Neutrophil depletion attenuated the interleukin-8 increase in the lung. Large Vt did not increase plasma interleukin-8 or tumor necrosis factor-alpha in plasma and bronchoalveolar lavage fluid. No expression of p-selectin or intercellular adhesion molecule-1 was observed. CONCLUSIONS: Cyclic overstretching of normal rabbit lungs with noninjurious large Vt produced neutrophil influx and interleukin-8 increase in bronchoalveolar lavage fluid. Production of pulmonary interleukin-8 by lung overstretch might require the interaction between resident lung cells and migrated neutrophils. This study suggests that large Vt ventilation potentiates the predisposed, subclinical lung injury, such as nosocomial pneumonia or aspiration of gastric contents.

Duke Scholars

Published In

Crit Care Med

DOI

ISSN

0090-3493

Publication Date

February 2004

Volume

32

Issue

2

Start / End Page

514 / 519

Location

United States

Related Subject Headings

  • Respiratory Distress Syndrome
  • Respiration, Artificial
  • Rabbits
  • Pulmonary Stretch Receptors
  • Neutrophils
  • Interleukin-8
  • Emergency & Critical Care Medicine
  • Animals
  • 1117 Public Health and Health Services
  • 1110 Nursing
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kotani, M., Kotani, T., Ishizaka, A., Fujishima, S., Koh, H., Tasaka, S., … Takeda, J. (2004). Neutrophil depletion attenuates interleukin-8 production in mild-overstretch ventilated normal rabbit lung. Crit Care Med, 32(2), 514–519. https://doi.org/10.1097/01.CCM.0000110677.16968.E4
Kotani, Mariko, Toru Kotani, Akitoshi Ishizaka, Seitaro Fujishima, Hidefumi Koh, Sadatomo Tasaka, Makoto Sawafuji, et al. “Neutrophil depletion attenuates interleukin-8 production in mild-overstretch ventilated normal rabbit lung.Crit Care Med 32, no. 2 (February 2004): 514–19. https://doi.org/10.1097/01.CCM.0000110677.16968.E4.
Kotani M, Kotani T, Ishizaka A, Fujishima S, Koh H, Tasaka S, et al. Neutrophil depletion attenuates interleukin-8 production in mild-overstretch ventilated normal rabbit lung. Crit Care Med. 2004 Feb;32(2):514–9.
Kotani, Mariko, et al. “Neutrophil depletion attenuates interleukin-8 production in mild-overstretch ventilated normal rabbit lung.Crit Care Med, vol. 32, no. 2, Feb. 2004, pp. 514–19. Pubmed, doi:10.1097/01.CCM.0000110677.16968.E4.
Kotani M, Kotani T, Ishizaka A, Fujishima S, Koh H, Tasaka S, Sawafuji M, Ikeda E, Moriyama K, Kotake Y, Morisaki H, Aikawa N, Ohashi A, Matsushima K, Huang Y-CT, Takeda J. Neutrophil depletion attenuates interleukin-8 production in mild-overstretch ventilated normal rabbit lung. Crit Care Med. 2004 Feb;32(2):514–519.

Published In

Crit Care Med

DOI

ISSN

0090-3493

Publication Date

February 2004

Volume

32

Issue

2

Start / End Page

514 / 519

Location

United States

Related Subject Headings

  • Respiratory Distress Syndrome
  • Respiration, Artificial
  • Rabbits
  • Pulmonary Stretch Receptors
  • Neutrophils
  • Interleukin-8
  • Emergency & Critical Care Medicine
  • Animals
  • 1117 Public Health and Health Services
  • 1110 Nursing