Effects of diethylstilbestrol on rat granulosa cell and thecal/interstitial cell transforming growth factor-beta 2 mRNA expression in vivo: analysis by reverse transcription-polymerase chain reaction.

Journal Article

Recent studies indicate that reproductive hormones may stimulate target cell growth via the actions of growth factors. In this study, reverse transcription-polymerase chain reaction analysis was used to assess the effects of in vivo diethylstilbestrol (DES) treatment on granulosa cell (GC) and thecal-interstitial cell (TIC) transforming growth factor (TGF)-beta 2 mRNA expression. In response to DES, GC expression of TGF-beta 2 mRNA was stimulated approximately 6.8-fold. By contrast, DES did not significantly alter TIC expression of TGF-beta 2 mRNA. Data were normalized relative to glyceraldehyde-3-phosphate dehydrogenase mRNA levels, which were unaffected by DES. These results extend our earlier findings, which demonstrated that GC and TIC expression of TGF-beta 2 mRNA is regulated by gonadotropins in vitro, to include steroidal regulation in vivo of intraovarian growth factor expression. Collectively, the present study and previous results support the following concepts: 1) intraovarian TGF-beta 2 mRNA expression is regulated by both gonadotropins and steroids, and 2) GC and TIC expression of TGF-beta 2 mRNA are differentially regulated.

Full Text

Duke Authors

Cited Authors

  • Mulheron, GW; Schomberg, DW

Published Date

  • April 1, 1992

Published In

Volume / Issue

  • 46 / 4

Start / End Page

  • 546 - 550

PubMed ID

  • 1576253

International Standard Serial Number (ISSN)

  • 0006-3363

Language

  • eng

Conference Location

  • United States