Rat granulosa cells express transforming growth factor-beta type 2 messenger ribonucleic acid which is regulatable by follicle-stimulating hormone in vitro.
Freshly harvested granulosa cells (GC) from diethylstilbestrol (DES)-treated rats were examined for the presence of transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 2 mRNA by Northern analysis. TGF-beta 1 mRNA was not detectable, but hybridization of total RNA with a radiolabeled TGF-beta 2 cDNA probe revealed two mRNA species (5.1 and 3.6 kb) indicative of TGF-beta 2 mRNA. In response to FSH (50 ng/ml), relative TGF-beta 2 mRNA concentrations in cultured GC were 54% of control levels. Furthermore, the conditioned culture medium from FSH-treated GC contained significantly lower (p less than 0.01) TGF-beta-like activity relative to controls. These results demonstrate that rat GC express TGF-beta 2 mRNA which is regulatable by FSH in vitro.
Duke Scholars
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- Tumor Cells, Cultured
- Transforming Growth Factors
- Rats
- RNA, Messenger
- Nucleic Acid Hybridization
- Humans
- Granulosa Cells
- Follicle Stimulating Hormone
- Female
- Endocrinology & Metabolism
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tumor Cells, Cultured
- Transforming Growth Factors
- Rats
- RNA, Messenger
- Nucleic Acid Hybridization
- Humans
- Granulosa Cells
- Follicle Stimulating Hormone
- Female
- Endocrinology & Metabolism