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Rat granulosa cells express transforming growth factor-beta type 2 messenger ribonucleic acid which is regulatable by follicle-stimulating hormone in vitro.

Publication ,  Journal Article
Mulheron, GW; Schomberg, DW
Published in: Endocrinology
March 1990

Freshly harvested granulosa cells (GC) from diethylstilbestrol (DES)-treated rats were examined for the presence of transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 2 mRNA by Northern analysis. TGF-beta 1 mRNA was not detectable, but hybridization of total RNA with a radiolabeled TGF-beta 2 cDNA probe revealed two mRNA species (5.1 and 3.6 kb) indicative of TGF-beta 2 mRNA. In response to FSH (50 ng/ml), relative TGF-beta 2 mRNA concentrations in cultured GC were 54% of control levels. Furthermore, the conditioned culture medium from FSH-treated GC contained significantly lower (p less than 0.01) TGF-beta-like activity relative to controls. These results demonstrate that rat GC express TGF-beta 2 mRNA which is regulatable by FSH in vitro.

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Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

March 1990

Volume

126

Issue

3

Start / End Page

1777 / 1779

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transforming Growth Factors
  • Rats
  • RNA, Messenger
  • Nucleic Acid Hybridization
  • Humans
  • Granulosa Cells
  • Follicle Stimulating Hormone
  • Female
  • Endocrinology & Metabolism
 

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Mulheron, G. W., & Schomberg, D. W. (1990). Rat granulosa cells express transforming growth factor-beta type 2 messenger ribonucleic acid which is regulatable by follicle-stimulating hormone in vitro. Endocrinology, 126(3), 1777–1779. https://doi.org/10.1210/endo-126-3-1777
Mulheron, G. W., and D. W. Schomberg. “Rat granulosa cells express transforming growth factor-beta type 2 messenger ribonucleic acid which is regulatable by follicle-stimulating hormone in vitro.Endocrinology 126, no. 3 (March 1990): 1777–79. https://doi.org/10.1210/endo-126-3-1777.
Mulheron, G. W., and D. W. Schomberg. “Rat granulosa cells express transforming growth factor-beta type 2 messenger ribonucleic acid which is regulatable by follicle-stimulating hormone in vitro.Endocrinology, vol. 126, no. 3, Mar. 1990, pp. 1777–79. Pubmed, doi:10.1210/endo-126-3-1777.
Journal cover image

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

March 1990

Volume

126

Issue

3

Start / End Page

1777 / 1779

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transforming Growth Factors
  • Rats
  • RNA, Messenger
  • Nucleic Acid Hybridization
  • Humans
  • Granulosa Cells
  • Follicle Stimulating Hormone
  • Female
  • Endocrinology & Metabolism