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Restricted expression of WT1 messenger ribonucleic acid in immature ovarian follicles: uniformity in mammalian and avian species and maintenance during reproductive senescence.

Publication ,  Journal Article
Chun, SY; McGee, EA; Hsu, SY; Minami, S; LaPolt, PS; Yao, HH; Bahr, JM; Gougeon, A; Schomberg, DW; Hsueh, AJ
Published in: Biol Reprod
February 1999

WT1 is a zinc finger protein with transcriptional repressor activity on several growth factor and growth factor receptor genes. In the ovary, a potential role for WT1 in the suppression of the development of immature follicles has been demonstrated. Here, gel retardation assays further showed that recombinant WT1 protein interacted with consensus DNA sequences in the inhibin-alpha gene promoter. We investigated the pattern of WT1 expression in a wide variety of species and also over the reproductive life span in rats. In chicken ovaries, Northern blot analysis revealed the presence of WT1 transcript in small healthy white follicles (1-5 mm in diameter) and its absence in small yellow (6-12 mm in diameter) or larger follicles (F1-F5). In pig and monkey ovaries, WT1 expression was limited to granulosa cells of preantral follicles, as shown by in situ hybridization analysis. In rats, Northern blot analyses demonstrated the presence of WT1 transcript in the ovaries of young (3-mo-old) and middle-aged (9-mo-old) rats on the proestrous day, with a decrease in old (12-mo-old) rats in persistent estrus. In situ hybridization analysis further suggested that the decrease in WT1 expression in aging ovaries was associated with fewer immature follicles. Thus, WT1 expression is restricted to immature follicles in diverse avian and mammalian species and over the reproductive life span in rats. These data demonstrated that WT1 is a marker for immature follicles and suggested a potential role of this transcriptional repressor in the slow growth of early follicles.

Duke Scholars

Published In

Biol Reprod

DOI

ISSN

0006-3363

Publication Date

February 1999

Volume

60

Issue

2

Start / End Page

365 / 373

Location

United States

Related Subject Headings

  • Zinc Fingers
  • WT1 Proteins
  • Transcription Factors
  • Swine
  • Species Specificity
  • Rats, Long-Evans
  • Rats
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Peptides
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chun, S. Y., McGee, E. A., Hsu, S. Y., Minami, S., LaPolt, P. S., Yao, H. H., … Hsueh, A. J. (1999). Restricted expression of WT1 messenger ribonucleic acid in immature ovarian follicles: uniformity in mammalian and avian species and maintenance during reproductive senescence. Biol Reprod, 60(2), 365–373. https://doi.org/10.1095/biolreprod60.2.365
Chun, S. Y., E. A. McGee, S. Y. Hsu, S. Minami, P. S. LaPolt, H. H. Yao, J. M. Bahr, A. Gougeon, D. W. Schomberg, and A. J. Hsueh. “Restricted expression of WT1 messenger ribonucleic acid in immature ovarian follicles: uniformity in mammalian and avian species and maintenance during reproductive senescence.Biol Reprod 60, no. 2 (February 1999): 365–73. https://doi.org/10.1095/biolreprod60.2.365.
Chun, S. Y., et al. “Restricted expression of WT1 messenger ribonucleic acid in immature ovarian follicles: uniformity in mammalian and avian species and maintenance during reproductive senescence.Biol Reprod, vol. 60, no. 2, Feb. 1999, pp. 365–73. Pubmed, doi:10.1095/biolreprod60.2.365.
Chun SY, McGee EA, Hsu SY, Minami S, LaPolt PS, Yao HH, Bahr JM, Gougeon A, Schomberg DW, Hsueh AJ. Restricted expression of WT1 messenger ribonucleic acid in immature ovarian follicles: uniformity in mammalian and avian species and maintenance during reproductive senescence. Biol Reprod. 1999 Feb;60(2):365–373.

Published In

Biol Reprod

DOI

ISSN

0006-3363

Publication Date

February 1999

Volume

60

Issue

2

Start / End Page

365 / 373

Location

United States

Related Subject Headings

  • Zinc Fingers
  • WT1 Proteins
  • Transcription Factors
  • Swine
  • Species Specificity
  • Rats, Long-Evans
  • Rats
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Peptides