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Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum.

Publication ,  Journal Article
Thomas, CJ; Brown, HL; Hawes, CR; Lee, BY; Min, MK; King, LA; Possee, RD
Published in: J Virol
December 1998

Confocal immunofluorescence microscopy was used to demonstrate that the Autographa californica nucleopolyhedrovirus (AcMNPV) chitinase was localized within the endoplasmic reticulum (ER) of virus-infected insect cells. This was consistent with removal of the signal peptide from the chitinase and an ER localization motif (KDEL) at the carboxyl end of the protein. Chitinase release from cells, a prerequisite for liquefaction of virus-infected insect larvae, appears to be aided by synthesis of the p10 protein. Deletion of p10 from the AcMNPV genome delayed the appearance of chitinase activity in the medium of virus-infected cells by 24 h and also delayed liquefaction of virus-infected Trichoplusia ni larvae by the same period.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

December 1998

Volume

72

Issue

12

Start / End Page

10207 / 10212

Location

United States

Related Subject Headings

  • Virology
  • Spodoptera
  • Protein Sorting Signals
  • Nucleopolyhedroviruses
  • Moths
  • Molecular Sequence Data
  • Microscopy, Immunoelectron
  • Microscopy, Fluorescence
  • Larva
  • Endoplasmic Reticulum
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Thomas, C. J., Brown, H. L., Hawes, C. R., Lee, B. Y., Min, M. K., King, L. A., & Possee, R. D. (1998). Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum. J Virol, 72(12), 10207–10212. https://doi.org/10.1128/JVI.72.12.10207-10212.1998
Thomas, C. J., H. L. Brown, C. R. Hawes, B. Y. Lee, M. K. Min, L. A. King, and R. D. Possee. “Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum.J Virol 72, no. 12 (December 1998): 10207–12. https://doi.org/10.1128/JVI.72.12.10207-10212.1998.
Thomas CJ, Brown HL, Hawes CR, Lee BY, Min MK, King LA, et al. Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum. J Virol. 1998 Dec;72(12):10207–12.
Thomas, C. J., et al. “Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum.J Virol, vol. 72, no. 12, Dec. 1998, pp. 10207–12. Pubmed, doi:10.1128/JVI.72.12.10207-10212.1998.
Thomas CJ, Brown HL, Hawes CR, Lee BY, Min MK, King LA, Possee RD. Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum. J Virol. 1998 Dec;72(12):10207–10212.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

December 1998

Volume

72

Issue

12

Start / End Page

10207 / 10212

Location

United States

Related Subject Headings

  • Virology
  • Spodoptera
  • Protein Sorting Signals
  • Nucleopolyhedroviruses
  • Moths
  • Molecular Sequence Data
  • Microscopy, Immunoelectron
  • Microscopy, Fluorescence
  • Larva
  • Endoplasmic Reticulum