Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum.

Journal Article (Journal Article)

Confocal immunofluorescence microscopy was used to demonstrate that the Autographa californica nucleopolyhedrovirus (AcMNPV) chitinase was localized within the endoplasmic reticulum (ER) of virus-infected insect cells. This was consistent with removal of the signal peptide from the chitinase and an ER localization motif (KDEL) at the carboxyl end of the protein. Chitinase release from cells, a prerequisite for liquefaction of virus-infected insect larvae, appears to be aided by synthesis of the p10 protein. Deletion of p10 from the AcMNPV genome delayed the appearance of chitinase activity in the medium of virus-infected cells by 24 h and also delayed liquefaction of virus-infected Trichoplusia ni larvae by the same period.

Full Text

Duke Authors

Cited Authors

  • Thomas, CJ; Brown, HL; Hawes, CR; Lee, BY; Min, MK; King, LA; Possee, RD

Published Date

  • December 1998

Published In

Volume / Issue

  • 72 / 12

Start / End Page

  • 10207 - 10212

PubMed ID

  • 9811762

Pubmed Central ID

  • PMC110568

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/JVI.72.12.10207-10212.1998


  • eng

Conference Location

  • United States