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Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium.

Publication ,  Journal Article
Hurd, WW; Fomin, VP; Natarajan, V; Brown, HL; Bigsby, RM; Singh, DM
Published in: Am J Obstet Gynecol
December 2000

OBJECTIVE: The aim of this study was to compare the distributions of protein kinase C isozymes in human nonpregnant and pregnant myometrial tissues and primary cell cultures. STUDY DESIGN: Myometrial tissues were obtained at hysterectomy from nonpregnant women and at cesarean delivery from women both before and during early labor at term. Western immunoblot analysis was performed on homogenates of myometrial tissues and primary cell cultures with monoclonal antibodies specific for protein kinase C isozymes. Redistribution and translocation of protein kinase C were examined by means of immunocytochemical methods. RESULTS: Nonpregnant myometrial tissues contained protein kinase C isozymes alpha, gamma, delta, mu, iota, and zeta but not beta(1), beta(2), theta, or epsilon. Pregnant myometrial tissues both before and during early labor contained the same protein kinase C isozymes and also beta(1) and beta(2). The protein kinase C isozyme distribution in primary myometrial cell cultures was identical to that in the myometrial tissues. Protein kinase C redistribution and translocation were demonstrated in these cultured myometrial cells. CONCLUSION: Both human myometrial tissues and primary cell cultures expressed a broad range of protein kinase C isozymes. Protein kinase C isozymes beta(1) and beta(2) were absent in nonpregnant myometrium but were induced during pregnancy. Labor at term did not alter protein kinase C isozyme expression.

Duke Scholars

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

December 2000

Volume

183

Issue

6

Start / End Page

1525 / 1531

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Tetradecanoylphorbol Acetate
  • Reference Values
  • Protein Kinase C
  • Pregnancy
  • Oxytocin
  • Obstetrics & Reproductive Medicine
  • Myometrium
  • Labor, Obstetric
  • Isoenzymes
 

Citation

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MLA
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Hurd, W. W., Fomin, V. P., Natarajan, V., Brown, H. L., Bigsby, R. M., & Singh, D. M. (2000). Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium. Am J Obstet Gynecol, 183(6), 1525–1531. https://doi.org/10.1067/mob.2000.107783
Hurd, W. W., V. P. Fomin, V. Natarajan, H. L. Brown, R. M. Bigsby, and D. M. Singh. “Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium.Am J Obstet Gynecol 183, no. 6 (December 2000): 1525–31. https://doi.org/10.1067/mob.2000.107783.
Hurd WW, Fomin VP, Natarajan V, Brown HL, Bigsby RM, Singh DM. Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium. Am J Obstet Gynecol. 2000 Dec;183(6):1525–31.
Hurd, W. W., et al. “Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium.Am J Obstet Gynecol, vol. 183, no. 6, Dec. 2000, pp. 1525–31. Pubmed, doi:10.1067/mob.2000.107783.
Hurd WW, Fomin VP, Natarajan V, Brown HL, Bigsby RM, Singh DM. Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium. Am J Obstet Gynecol. 2000 Dec;183(6):1525–1531.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

December 2000

Volume

183

Issue

6

Start / End Page

1525 / 1531

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Tetradecanoylphorbol Acetate
  • Reference Values
  • Protein Kinase C
  • Pregnancy
  • Oxytocin
  • Obstetrics & Reproductive Medicine
  • Myometrium
  • Labor, Obstetric
  • Isoenzymes