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Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis.

Publication ,  Journal Article
Brown, HL; Fuller, DD; Jasper, LT; Davis, TE; Wright, JD
Published in: Infect Dis Obstet Gynecol
2004

OBJECTIVE: To compare the Affirm VPIII Microbial Identification Test for detection and identification of Candida species, Gardnerella vaginalis and Trichomonas vaginalis to clinical and microscopic criteria commonly used to diagnose vaginitis. METHODS: Women that were symptomatic for vaginitis/vaginosis and asymptomatic women being seen for routine obstetric or gynecological care were included in this study. Women treated with antibiotics or antifungals within one week or women who had douched within 24 hours were excluded. Two vaginal swab specimens were simultaneously obtained from each patient, one swab was placed in sterile physiological saline for immediate microscopic wet mount examination and KOH testing. The other swab was placed in the Affirm collection tube for Affirm VPIII testing based on previously demonstrated methods. RESULTS: The Affirm assay was significantly more likely to identify Gardnerella and Candida than wet mount. 190 (45%) were positive for Gardnerella by Affirm compared to 58 (14%) by wet mount; 45 (11%) were positive for Candida by Affirm compared to 31 (7%) by wet mount; and 30 (7%) were positive for Trichomonas by Affirm compared to 23 (5%) by wet mount. Symptomatic women were significantly more likely to be positive by Affirm only (23% vs. 10%), wet mount only (3% vs. 2%) or Affirm and wet mount (15% vs. 1%). Asymptomatic women were significantly more likely to be negative for Affirm and wet mount (43% vs. 5%). CONCLUSIONS: The Affirm VPIII test is a more sensitive diagnostic test for detection and identification of symptomatic vaginitis/vaginosis than conventional clinical examination and wet mount testing.

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Published In

Infect Dis Obstet Gynecol

DOI

ISSN

1064-7449

Publication Date

2004

Volume

12

Issue

1

Start / End Page

17 / 21

Location

Egypt

Related Subject Headings

  • Vaginosis, Bacterial
  • Vaginal Smears
  • Vaginal Diseases
  • Trichomonas vaginalis
  • Trichomonas Vaginitis
  • Sensitivity and Specificity
  • Reagent Kits, Diagnostic
  • Predictive Value of Tests
  • Obstetrics & Reproductive Medicine
  • Indiana
 

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Brown, H. L., Fuller, D. D., Jasper, L. T., Davis, T. E., & Wright, J. D. (2004). Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis. Infect Dis Obstet Gynecol, 12(1), 17–21. https://doi.org/10.1080/1064744042000210375
Brown, Haywood L., Deanna D. Fuller, Lori T. Jasper, Thomas E. Davis, and Justin D. Wright. “Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis.Infect Dis Obstet Gynecol 12, no. 1 (2004): 17–21. https://doi.org/10.1080/1064744042000210375.
Brown, Haywood L., et al. “Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis.Infect Dis Obstet Gynecol, vol. 12, no. 1, 2004, pp. 17–21. Pubmed, doi:10.1080/1064744042000210375.
Journal cover image

Published In

Infect Dis Obstet Gynecol

DOI

ISSN

1064-7449

Publication Date

2004

Volume

12

Issue

1

Start / End Page

17 / 21

Location

Egypt

Related Subject Headings

  • Vaginosis, Bacterial
  • Vaginal Smears
  • Vaginal Diseases
  • Trichomonas vaginalis
  • Trichomonas Vaginitis
  • Sensitivity and Specificity
  • Reagent Kits, Diagnostic
  • Predictive Value of Tests
  • Obstetrics & Reproductive Medicine
  • Indiana