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A novel conditionally replicative adenovirus vector targeting telomerase-positive tumor cells.

Publication ,  Journal Article
Huang, Q; Zhang, X; Wang, H; Yan, B; Kirkpatrick, J; Dewhrist, MW; Li, C-Y
Published in: Clin Cancer Res
February 15, 2004

PURPOSE: To develop a novel conditionally replicative adenovirus vector that targets telomerase-positive cancer cells. EXPERIMENTAL DESIGN: A telomerase gene-derived promoter was used to control the expression of the E1a gene so that the E1a gene is only expressed in telomerase-positive tumor cells. In addition, a reporter gene was also engineered into the vector so that its infection and replication can be monitored easily. RESULTS: A novel recombinant adenovirus vector that could selectively replicate in telomerase-positive cancer cells was made successfully. This vector showed active replication in a panel of cancer cells and minimal replication in normal human fibroblast or epithelial cells. The recombinant vector could effectively lyse various cultured tumor cells even at very low multiplicity of infection. The replication efficiency in tumor cells is over 10(3)-fold more than normal fibroblast and epithelial cells. In s.c. tumor models, the newly developed telomerase-selective adenovirus vectors exhibited significantly more virus replication and reporter gene expression. CONCLUSIONS: The telomerase-targeted adenovirus vector has significant potential as an oncolytic virus as well as a tumor-specific therapeutic gene delivery vehicle.

Duke Scholars

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Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

February 15, 2004

Volume

10

Issue

4

Start / End Page

1439 / 1445

Location

United States

Related Subject Headings

  • Time Factors
  • Telomerase
  • Prostatic Neoplasms
  • Prostate
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Male
  • Luminescent Proteins
  • Humans
  • Genetic Vectors
 

Citation

APA
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ICMJE
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Huang, Q., Zhang, X., Wang, H., Yan, B., Kirkpatrick, J., Dewhrist, M. W., & Li, C.-Y. (2004). A novel conditionally replicative adenovirus vector targeting telomerase-positive tumor cells. Clin Cancer Res, 10(4), 1439–1445. https://doi.org/10.1158/1078-0432.ccr-03-0122
Huang, Qian, Xiuwu Zhang, He Wang, Bin Yan, John Kirkpatrick, Mark W. Dewhrist, and Chuan-Yuan Li. “A novel conditionally replicative adenovirus vector targeting telomerase-positive tumor cells.Clin Cancer Res 10, no. 4 (February 15, 2004): 1439–45. https://doi.org/10.1158/1078-0432.ccr-03-0122.
Huang Q, Zhang X, Wang H, Yan B, Kirkpatrick J, Dewhrist MW, et al. A novel conditionally replicative adenovirus vector targeting telomerase-positive tumor cells. Clin Cancer Res. 2004 Feb 15;10(4):1439–45.
Huang, Qian, et al. “A novel conditionally replicative adenovirus vector targeting telomerase-positive tumor cells.Clin Cancer Res, vol. 10, no. 4, Feb. 2004, pp. 1439–45. Pubmed, doi:10.1158/1078-0432.ccr-03-0122.
Huang Q, Zhang X, Wang H, Yan B, Kirkpatrick J, Dewhrist MW, Li C-Y. A novel conditionally replicative adenovirus vector targeting telomerase-positive tumor cells. Clin Cancer Res. 2004 Feb 15;10(4):1439–1445.

Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

February 15, 2004

Volume

10

Issue

4

Start / End Page

1439 / 1445

Location

United States

Related Subject Headings

  • Time Factors
  • Telomerase
  • Prostatic Neoplasms
  • Prostate
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Male
  • Luminescent Proteins
  • Humans
  • Genetic Vectors