Down-regulation of the orphan nuclear receptor ROR gamma t is essential for T lymphocyte maturation.
Journal Article (Journal Article)
Thymocyte development is a tightly regulated process. CD4+CD8+ double-positive (DP) immature thymocytes exhibit distinct phenotypic features from mature T cells; they express only 10% of surface TCR that are found on mature T cells and do not proliferate and produce IL-2 in response to stimulation. In this report we show that transgenic expression of the orphan nuclear receptor ROR gamma t in mature T cells down-regulates their surface TCR expression. The ROR gamma t transgene inhibits IL-2 production by mature T cells, and this inhibition may be partially due to the inhibitory effect of ROR gamma t on c-Rel transcription. Furthermore, ectopic expression of ROR gamma t inhibits the proliferation of mature and immature T cells. These results, together with its predominant expression in DP thymocytes, suggest that ROR gamma t controls these distinct phenotypic features of DP thymocytes. Our data suggest that down-regulation of ROR gamma t expression in thymocytes is essential for their maturation.
- He, YW; Beers, C; Deftos, ML; Ojala, EW; Forbush, KA; Bevan, MJ
- June 1, 2000
Volume / Issue
- 164 / 11
Start / End Page
- 5668 - 5674
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)
- United States