The common gamma-chain of cytokine receptors regulates intrathymic T cell development at multiple stages.

Published

Journal Article

Signaling through the common gamma chain (gamma c), a subunit of the receptors for IL-2, -4, -7, -9, and -15, is critical for lymphocyte development, with the IL-7/IL-7R representing one important interaction. To investigate the stages of intrathymic T cell development that are dependent on gamma c and to determine whether gamma c controls T cell development solely as a component of the IL-7R, intrathymic T cell development was compared in IL-7R alpha-deficient mice and anti-gamma c-treated chimeric mice reconstituted with bone marrow and purified pro-T cells. In the presence of anti-gamma c, each of four phenotypically distinguishable stages of CD4- CD8- thymocytes failed to reconstitute T cell development, suggesting that each of these subsets of pro-T cells required gamma c for their differentiation and/or growth. Reconstitution of anti-gamma c-treated chimeric mice with bone marrow from IL-7R alpha-deficient mice indicated that IL-7R only partially contributed to intrathymic T cell development. Furthermore, when compared with IL-7R-deficient mice, anti-gamma c chimeric and gamma c-deficient mice exhibited a distinct phenotypic pattern of pro-T cell development. Collectively, these results indicate that several gamma c-sharing cytokines may contribute to T cell development in the thymus and suggest that one of these cytokines may be novel.

Full Text

Duke Authors

Cited Authors

  • He, YW; Nakajima, H; Leonard, WJ; Adkins, B; Malek, TR

Published Date

  • March 15, 1997

Published In

Volume / Issue

  • 158 / 6

Start / End Page

  • 2592 - 2599

PubMed ID

  • 9058791

Pubmed Central ID

  • 9058791

International Standard Serial Number (ISSN)

  • 0022-1767

Language

  • eng

Conference Location

  • United States