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In vivo catabolism of alpha 1-proteinase inhibitor-trypsin, antithrombin III-thrombin and alpha 2-macroglobulin-methylamine.

Publication ,  Journal Article
Fuchs, HE; Shifman, MA; Pizzo, SV
Published in: Biochim Biophys Acta
May 27, 1982

The clearances of 125I-labeled alpha 1-proteinase inhibitor-trypsin, antithrombin III-thrombin and alpha 2-macroglobulin-methylamine (CH3NH2) were compared in our previously described mouse model. alpha 1-Proteinase inhibitor-trypsin cleared with a t 1/2 of 20 min, antithrombin III-thrombin of 7 min and 125I-labeled alpha 2-macroglobulin-methylamine of 2 min. Competition studies were performed to determine whether one or several pathways clear these three ligands. The clearance of 125I-labeled alpha 1-proteinase inhibitor-trypsin and 125I-labeled antithrombin III-thrombin was blocked by large molar excesses of either ligand, but not by alpha 2-macroglobulin-methylamine. The clearance of 125I-labeled alpha 2-macroglobulin-methylamine can be blocked by a large molar excesses of unlabeled alpha 2-macroglobulin-methylamine but not by alpha 1-proteinase inhibitor-trypsin. These studies demonstrate that the clearance of alpha 1-proteinase inhibitor-trypsin complexes is independent of alpha 2-macroglobulin-methylamine and utilizes the same pathway which is involved in the clearance of antithrombin III-thrombin complexes.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

May 27, 1982

Volume

716

Issue

2

Start / End Page

151 / 157

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • alpha 1-Antitrypsin
  • Trypsin
  • Tissue Distribution
  • Thrombin
  • Mice
  • Methylamines
  • Female
  • Electrophoresis, Polyacrylamide Gel
  • Binding, Competitive
 

Citation

APA
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ICMJE
MLA
NLM
Fuchs, H. E., Shifman, M. A., & Pizzo, S. V. (1982). In vivo catabolism of alpha 1-proteinase inhibitor-trypsin, antithrombin III-thrombin and alpha 2-macroglobulin-methylamine. Biochim Biophys Acta, 716(2), 151–157. https://doi.org/10.1016/0304-4165(82)90263-x
Fuchs, H. E., M. A. Shifman, and S. V. Pizzo. “In vivo catabolism of alpha 1-proteinase inhibitor-trypsin, antithrombin III-thrombin and alpha 2-macroglobulin-methylamine.Biochim Biophys Acta 716, no. 2 (May 27, 1982): 151–57. https://doi.org/10.1016/0304-4165(82)90263-x.
Fuchs, H. E., et al. “In vivo catabolism of alpha 1-proteinase inhibitor-trypsin, antithrombin III-thrombin and alpha 2-macroglobulin-methylamine.Biochim Biophys Acta, vol. 716, no. 2, May 1982, pp. 151–57. Pubmed, doi:10.1016/0304-4165(82)90263-x.
Fuchs HE, Shifman MA, Pizzo SV. In vivo catabolism of alpha 1-proteinase inhibitor-trypsin, antithrombin III-thrombin and alpha 2-macroglobulin-methylamine. Biochim Biophys Acta. 1982 May 27;716(2):151–157.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

May 27, 1982

Volume

716

Issue

2

Start / End Page

151 / 157

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • alpha 1-Antitrypsin
  • Trypsin
  • Tissue Distribution
  • Thrombin
  • Mice
  • Methylamines
  • Female
  • Electrophoresis, Polyacrylamide Gel
  • Binding, Competitive