Requirement of the prolyl isomerase Pin1 for the replication checkpoint.

Journal Article (Journal Article)

The peptidyl-prolyl isomerase Pin1 has been implicated in regulating cell cycle progression. Pin1 was found to be required for the DNA replication checkpoint in Xenopus laevis. Egg extracts depleted of Pin1 inappropriately transited from the G2 to the M phase of the cell cycle in the presence of the DNA replication inhibitor aphidicolin. This defect in replication checkpoint function was reversed after the addition of recombinant wild-type Pin1, but not an isomerase-inactive mutant, to the depleted extract. Premature mitotic entry in the absence of Pin1 was accompanied by hyperphosphorylation of Cdc25, activation of Cdc2/cyclin B, and generation of epitopes recognized by the mitotic phosphoprotein antibody, MPM-2. Therefore, Pin1 appears to be required for the checkpoint delaying the onset of mitosis in response to incomplete replication.

Full Text

Duke Authors

Cited Authors

  • Winkler, KE; Swenson, KI; Kornbluth, S; Means, AR

Published Date

  • March 3, 2000

Published In

Volume / Issue

  • 287 / 5458

Start / End Page

  • 1644 - 1647

PubMed ID

  • 10698738

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.287.5458.1644


  • eng

Conference Location

  • United States