Presence and indirect immunofluorescent localization of calmodulin in Paramecium tetraurelia.

Published

Journal Article

In this paper we demonstrate the presence and localization of calmodulin, a calcium-dependent regulatory protein, in the ciliated protozoan Paramecium tetraurelia. Calmodulin is demonstrated by several criteria: (a) the ability of whole cell Paramecium extracts to stimulate mammalian phosphodiesterase activity, (b) the presence of an acidic, thermostable, 17,000-dalton polypeptide whose mobility shifts in SDS polyacrylamide gel electrophoresis in the presence of Ca2+, and (c) the affinity of antibodies against mammalian calmodulin for a Paramecium component as demonstrated by both indirect immunofluorescent localization and radioimmunoassay. Indirect immunofluorescence studies reveal that Paramecium calmodulin is distributed in three distinct regions of the cell, i.e., (a) large, spherical cytoplasmic organelles representing perhaps the food vacuoles or other vacuolar inclusions of the cell, (b) along the entire length of oral and somatic cilia, and (c) along a linear punctate pattern corresponding to the kinetics (basal bodies) of the cell.

Full Text

Duke Authors

Cited Authors

  • Maihle, NJ; Dedman, JR; Means, AR; Chafouleas, JG; Satir, BH

Published Date

  • June 1981

Published In

Volume / Issue

  • 89 / 3

Start / End Page

  • 695 - 699

PubMed ID

  • 7019219

Pubmed Central ID

  • 7019219

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.89.3.695

Language

  • eng

Conference Location

  • United States