Characterization of a spectrophotometric assay for cAMP phosphodiesterase.
Publication
, Journal Article
Dedman, JR; Means, AR
Published in: J Cyclic Nucleotide Res
April 1977
The hydrolysis of cAMP can be monitored spectrophotometrically through the conversion of 5' AMP to 5' IMP using a specific 5' AMP amino-hydrolase (EC3.5.4.6). The optical properties and extinction coefficient differences of these compounds have been quantitatively determined. Phosphodiesterase assayed in this manner is linear with respect to time and enzyme concentration, and is more reliable than conventional assay procedures. Due to the high specificity of the assay system phosphodiesterase can be selectively assayed in unfractionated cytosol. The assay, when conducted on a conventional spectrophotometer, can detect the hydrolysis of 0.5 nmoles cAMP per min.
Duke Scholars
Published In
J Cyclic Nucleotide Res
ISSN
0095-1544
Publication Date
April 1977
Volume
3
Issue
2
Start / End Page
139 / 152
Location
United States
Related Subject Headings
- Spectrophotometry
- Rats
- Phosphoric Diester Hydrolases
- Myocardium
- Male
- Inosine Nucleotides
- Edetic Acid
- Cyclic AMP
- Cattle
- Brain
Citation
APA
Chicago
ICMJE
MLA
NLM
Dedman, J. R., & Means, A. R. (1977). Characterization of a spectrophotometric assay for cAMP phosphodiesterase. J Cyclic Nucleotide Res, 3(2), 139–152.
Dedman, J. R., and A. R. Means. “Characterization of a spectrophotometric assay for cAMP phosphodiesterase.” J Cyclic Nucleotide Res 3, no. 2 (April 1977): 139–52.
Dedman JR, Means AR. Characterization of a spectrophotometric assay for cAMP phosphodiesterase. J Cyclic Nucleotide Res. 1977 Apr;3(2):139–52.
Dedman, J. R., and A. R. Means. “Characterization of a spectrophotometric assay for cAMP phosphodiesterase.” J Cyclic Nucleotide Res, vol. 3, no. 2, Apr. 1977, pp. 139–52.
Dedman JR, Means AR. Characterization of a spectrophotometric assay for cAMP phosphodiesterase. J Cyclic Nucleotide Res. 1977 Apr;3(2):139–152.
Published In
J Cyclic Nucleotide Res
ISSN
0095-1544
Publication Date
April 1977
Volume
3
Issue
2
Start / End Page
139 / 152
Location
United States
Related Subject Headings
- Spectrophotometry
- Rats
- Phosphoric Diester Hydrolases
- Myocardium
- Male
- Inosine Nucleotides
- Edetic Acid
- Cyclic AMP
- Cattle
- Brain