Characterization of a spectrophotometric assay for cAMP phosphodiesterase.

Published

Journal Article

The hydrolysis of cAMP can be monitored spectrophotometrically through the conversion of 5' AMP to 5' IMP using a specific 5' AMP amino-hydrolase (EC3.5.4.6). The optical properties and extinction coefficient differences of these compounds have been quantitatively determined. Phosphodiesterase assayed in this manner is linear with respect to time and enzyme concentration, and is more reliable than conventional assay procedures. Due to the high specificity of the assay system phosphodiesterase can be selectively assayed in unfractionated cytosol. The assay, when conducted on a conventional spectrophotometer, can detect the hydrolysis of 0.5 nmoles cAMP per min.

Full Text

Duke Authors

Cited Authors

  • Dedman, JR; Means, AR

Published Date

  • April 1, 1977

Published In

Volume / Issue

  • 3 / 2

Start / End Page

  • 139 - 152

PubMed ID

  • 195985

Pubmed Central ID

  • 195985

International Standard Serial Number (ISSN)

  • 0095-1544

Language

  • eng