Mapping of calmodulin-binding domain of Ca2+/calmodulin-dependent protein kinase II from rat brain.
Journal Article (Journal Article)
Recent molecular cloning experiments have identified a 25 amino-acid region as the calmodulin-binding domain of the alpha-subunit of rat brain Ca2+/calmodulin-dependent multifunctional protein kinase II (CaM-K II). Synthetic peptides, derived from the deduced amino-acid sequence encompassing this region, were examined for their ability to bind calmodulin in a calcium dependent manner and to inhibit the Ca2+/calmodulin-dependent autophosphorylation of CaM-K II. Comparison of these structure-function relationships highlighted a region of 5 amino-acids, which was essential for calmodulin interaction and inhibition of kinase activity. This region demonstrated some homology with other calmodulin-binding peptides, and may represent a key site of interaction of the kinase with calmodulin. These analyses provide additional insight into the molecular mechanism underlying the Ca2+ regulation of CaM-K II.
Full Text
Duke Authors
Cited Authors
- Hanley, RM; Means, AR; Kemp, BE; Shenolikar, S
Published Date
- April 15, 1988
Published In
Volume / Issue
- 152 / 1
Start / End Page
- 122 - 128
PubMed ID
- 2833884
International Standard Serial Number (ISSN)
- 0006-291X
Digital Object Identifier (DOI)
- 10.1016/s0006-291x(88)80688-0
Language
- eng
Conference Location
- United States