Mapping of calmodulin-binding domain of Ca2+/calmodulin-dependent protein kinase II from rat brain.

Journal Article (Journal Article)

Recent molecular cloning experiments have identified a 25 amino-acid region as the calmodulin-binding domain of the alpha-subunit of rat brain Ca2+/calmodulin-dependent multifunctional protein kinase II (CaM-K II). Synthetic peptides, derived from the deduced amino-acid sequence encompassing this region, were examined for their ability to bind calmodulin in a calcium dependent manner and to inhibit the Ca2+/calmodulin-dependent autophosphorylation of CaM-K II. Comparison of these structure-function relationships highlighted a region of 5 amino-acids, which was essential for calmodulin interaction and inhibition of kinase activity. This region demonstrated some homology with other calmodulin-binding peptides, and may represent a key site of interaction of the kinase with calmodulin. These analyses provide additional insight into the molecular mechanism underlying the Ca2+ regulation of CaM-K II.

Full Text

Duke Authors

Cited Authors

  • Hanley, RM; Means, AR; Kemp, BE; Shenolikar, S

Published Date

  • April 15, 1988

Published In

Volume / Issue

  • 152 / 1

Start / End Page

  • 122 - 128

PubMed ID

  • 2833884

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/s0006-291x(88)80688-0


  • eng

Conference Location

  • United States