Stability of nuclear triiodothyronine binding sites.

Journal Article

Rat liver nuclei have recently been shown to possess limited, high affinity binding sites for triiodothyronine, only a fraction of which are believed to be filled in vivo. We have found that preincubation of nuclei in the absence of added triiodothyronine leads to a rapid decrease in binding capacity. This loss of binding sites is considerably faster at 37 C than at 4 C. On the other hand, once binding equilibrium is reached between nuclear bound and free triiodothyronine the level of binding does not change during the time in which a considerable loss of binding had occurred when nuclei were preincubated in the absence of hormone. Also the rate of dissociation of triiodothyronine from nuclei is slower than the rate of loss of binding sites during preincubation in the absence of added hormone. Thus, it appears as if unfilled sites are being lost during the preincubation period. Administration of cycloheximide or actinomycin D, inhibitors of protein and RNA synthesis, respectively, to rats caused a decrease in the amount of triiodothyronine which could be bound in the in vitro binding assay to liver nuclei.

Full Text

Duke Authors

Cited Authors

  • Jaffe, RC; Means, AR

Published Date

  • August 1, 1977

Published In

Volume / Issue

  • 101 / 2

Start / End Page

  • 447 - 452

PubMed ID

  • 195793

International Standard Serial Number (ISSN)

  • 0013-7227

Digital Object Identifier (DOI)

  • 10.1210/endo-101-2-447

Language

  • eng

Conference Location

  • United States