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Stability of nuclear triiodothyronine binding sites.

Publication ,  Journal Article
Jaffe, RC; Means, AR
Published in: Endocrinology
August 1977

Rat liver nuclei have recently been shown to possess limited, high affinity binding sites for triiodothyronine, only a fraction of which are believed to be filled in vivo. We have found that preincubation of nuclei in the absence of added triiodothyronine leads to a rapid decrease in binding capacity. This loss of binding sites is considerably faster at 37 C than at 4 C. On the other hand, once binding equilibrium is reached between nuclear bound and free triiodothyronine the level of binding does not change during the time in which a considerable loss of binding had occurred when nuclei were preincubated in the absence of hormone. Also the rate of dissociation of triiodothyronine from nuclei is slower than the rate of loss of binding sites during preincubation in the absence of added hormone. Thus, it appears as if unfilled sites are being lost during the preincubation period. Administration of cycloheximide or actinomycin D, inhibitors of protein and RNA synthesis, respectively, to rats caused a decrease in the amount of triiodothyronine which could be bound in the in vitro binding assay to liver nuclei.

Duke Scholars

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

August 1977

Volume

101

Issue

2

Start / End Page

447 / 452

Location

United States

Related Subject Headings

  • Triiodothyronine
  • Time Factors
  • Receptors, Cell Surface
  • Rats
  • Male
  • Liver
  • Endocrinology & Metabolism
  • Dactinomycin
  • Cycloheximide
  • Cell Nucleus
 

Citation

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MLA
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Jaffe, R. C., & Means, A. R. (1977). Stability of nuclear triiodothyronine binding sites. Endocrinology, 101(2), 447–452. https://doi.org/10.1210/endo-101-2-447
Jaffe, R. C., and A. R. Means. “Stability of nuclear triiodothyronine binding sites.Endocrinology 101, no. 2 (August 1977): 447–52. https://doi.org/10.1210/endo-101-2-447.
Jaffe RC, Means AR. Stability of nuclear triiodothyronine binding sites. Endocrinology. 1977 Aug;101(2):447–52.
Jaffe, R. C., and A. R. Means. “Stability of nuclear triiodothyronine binding sites.Endocrinology, vol. 101, no. 2, Aug. 1977, pp. 447–52. Pubmed, doi:10.1210/endo-101-2-447.
Jaffe RC, Means AR. Stability of nuclear triiodothyronine binding sites. Endocrinology. 1977 Aug;101(2):447–452.
Journal cover image

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

August 1977

Volume

101

Issue

2

Start / End Page

447 / 452

Location

United States

Related Subject Headings

  • Triiodothyronine
  • Time Factors
  • Receptors, Cell Surface
  • Rats
  • Male
  • Liver
  • Endocrinology & Metabolism
  • Dactinomycin
  • Cycloheximide
  • Cell Nucleus