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Calmodulin-microtubule association in cultured mammalian cells.

Publication ,  Journal Article
Deery, WJ; Means, AR; Brinkley, BR
Published in: J Cell Biol
March 1984

A Triton X-100-lysed cell system has been used to identify calmodulin on the cytoskeleton of 3T3 and transformed SV3T3 cells. By indirect immunofluorescence, calmodulin was found to be associated with both the cytoplasmic microtubule complex and the centrosomes. A number of cytoplasmic microtubules more resistant to disassembly upon either cold (0-4 degrees C) or hypotonic treatment, as well as following dilution have been identified. Most of the stable microtubules appeared to be associated with the centrosome at one end and with the plasma membrane at the other end. These microtubules could be induced to depolymerize, however, by micromolar Ca++ concentrations. These data suggest that, by interacting directly with the microtubule, calmodulin may influence microtubule assembly and ensure the Ca++-sensitivity of both mitotic and cytoplasmic microtubules.

Duke Scholars

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

March 1984

Volume

98

Issue

3

Start / End Page

904 / 910

Location

United States

Related Subject Headings

  • Tubulin
  • Protein Binding
  • Microtubules
  • Mice
  • Fluorescent Antibody Technique
  • Developmental Biology
  • Cold Temperature
  • Cells, Cultured
  • Calmodulin
  • Calcium
 

Citation

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Deery, W. J., Means, A. R., & Brinkley, B. R. (1984). Calmodulin-microtubule association in cultured mammalian cells. J Cell Biol, 98(3), 904–910. https://doi.org/10.1083/jcb.98.3.904
Deery, W. J., A. R. Means, and B. R. Brinkley. “Calmodulin-microtubule association in cultured mammalian cells.J Cell Biol 98, no. 3 (March 1984): 904–10. https://doi.org/10.1083/jcb.98.3.904.
Deery WJ, Means AR, Brinkley BR. Calmodulin-microtubule association in cultured mammalian cells. J Cell Biol. 1984 Mar;98(3):904–10.
Deery, W. J., et al. “Calmodulin-microtubule association in cultured mammalian cells.J Cell Biol, vol. 98, no. 3, Mar. 1984, pp. 904–10. Pubmed, doi:10.1083/jcb.98.3.904.
Deery WJ, Means AR, Brinkley BR. Calmodulin-microtubule association in cultured mammalian cells. J Cell Biol. 1984 Mar;98(3):904–910.

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

March 1984

Volume

98

Issue

3

Start / End Page

904 / 910

Location

United States

Related Subject Headings

  • Tubulin
  • Protein Binding
  • Microtubules
  • Mice
  • Fluorescent Antibody Technique
  • Developmental Biology
  • Cold Temperature
  • Cells, Cultured
  • Calmodulin
  • Calcium