Calmodulin-microtubule association in cultured mammalian cells.

Journal Article

A Triton X-100-lysed cell system has been used to identify calmodulin on the cytoskeleton of 3T3 and transformed SV3T3 cells. By indirect immunofluorescence, calmodulin was found to be associated with both the cytoplasmic microtubule complex and the centrosomes. A number of cytoplasmic microtubules more resistant to disassembly upon either cold (0-4 degrees C) or hypotonic treatment, as well as following dilution have been identified. Most of the stable microtubules appeared to be associated with the centrosome at one end and with the plasma membrane at the other end. These microtubules could be induced to depolymerize, however, by micromolar Ca++ concentrations. These data suggest that, by interacting directly with the microtubule, calmodulin may influence microtubule assembly and ensure the Ca++-sensitivity of both mitotic and cytoplasmic microtubules.

Full Text

Duke Authors

Cited Authors

  • Deery, WJ; Means, AR; Brinkley, BR

Published Date

  • March 1, 1984

Published In

Volume / Issue

  • 98 / 3

Start / End Page

  • 904 - 910

PubMed ID

  • 6365929

International Standard Serial Number (ISSN)

  • 0021-9525

Language

  • eng

Conference Location

  • United States