Functional analysis of a complementary DNA for the 50-kilodalton subunit of calmodulin kinase II.

Journal Article (Journal Article)

The calcium-calmodulin-dependent protein kinase II is a major component of brain synaptic junctions and has been proposed to play a variety of important roles in brain function. A complementary DNA representing a portion of the smaller 50-kilodalton subunit of the rat brain enzyme has been cloned and sequenced. The calmodulin-binding region has been identified and a synthetic analog prepared that binds calmodulin with high affinity in the presence of calcium. Like the 50-kilodalton kinase polypeptide, the concentration of the messenger RNA varies both neuroanatomically and during postnatal development of the brain. The broad tissue and species cross-reactivity of the complementary DNA suggests that the 50-kilodalton subunit found in rat brain is evolutionarily conserved and is the product of a single gene.

Full Text

Duke Authors

Cited Authors

  • Hanley, RM; Means, AR; Ono, T; Kemp, BE; Burgin, KE; Waxham, N; Kelly, PT

Published Date

  • July 17, 1987

Published In

Volume / Issue

  • 237 / 4812

Start / End Page

  • 293 - 297

PubMed ID

  • 3037704

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.3037704


  • eng

Conference Location

  • United States