Functional analysis of a complementary DNA for the 50-kilodalton subunit of calmodulin kinase II.
The calcium-calmodulin-dependent protein kinase II is a major component of brain synaptic junctions and has been proposed to play a variety of important roles in brain function. A complementary DNA representing a portion of the smaller 50-kilodalton subunit of the rat brain enzyme has been cloned and sequenced. The calmodulin-binding region has been identified and a synthetic analog prepared that binds calmodulin with high affinity in the presence of calcium. Like the 50-kilodalton kinase polypeptide, the concentration of the messenger RNA varies both neuroanatomically and during postnatal development of the brain. The broad tissue and species cross-reactivity of the complementary DNA suggests that the 50-kilodalton subunit found in rat brain is evolutionarily conserved and is the product of a single gene.
Duke Scholars
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Related Subject Headings
- Species Specificity
- Rats
- RNA, Messenger
- Protein Kinases
- General Science & Technology
- DNA
- Cloning, Molecular
- Calcium-Calmodulin-Dependent Protein Kinases
- Brain
- Biological Assay
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Species Specificity
- Rats
- RNA, Messenger
- Protein Kinases
- General Science & Technology
- DNA
- Cloning, Molecular
- Calcium-Calmodulin-Dependent Protein Kinases
- Brain
- Biological Assay