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The structural organization of the chicken calmodulin gene.

Publication ,  Journal Article
Simmen, RC; Tanaka, T; Ts'ui, KF; Putkey, JA; Scott, MJ; Lai, EC; Means, AR
Published in: J Biol Chem
January 25, 1985

The structural organization of the entire chicken calmodulin (CaM) gene was determined by analysis of overlapping genomic clones obtained from Charon 4A and cosmid DNA libraries. These clones together span 39 kilobases of chicken genomic DNA. The CaM gene is 12 kilobases long and contains 8 exons interrupted by introns of highly variable size. The first intron (A) is only 19 base pairs (bp) long and divides the 5' untranslated region. Intron B separates the ATG from the first nucleotide of the triplet which encodes the NH2-terminal amino acid of CaM (Ala) whereas intron C separates the triplets encoding amino acids 10-11. Introns D, F, and G interrupt the Ca2+ binding subdomains II, III, and IV of CaM whereas intron E is localized in the linker region between the highly homologous NH2- and COOH-terminal halves of the protein. Primer extension studies using chicken brain poly(A+) mRNA and a fragment from the 5' untranslated region of a CaM cDNA identified the presumptive transcription initiation site (cap site) of the mRNA to be 103 bp 5' from the initiation condon ATG. A consensus sequence TATTTAA was localized 29 bp 5' from this cap site while a CCAAT sequence was located further 5' at position -58 bp. The structure of the CaM gene is strikingly similar to genes that encode other Ca2+ binding proteins from sea urchin, chicken, rat, and quail. These data suggest a conservation of genome organization related to the calcium binding and regulatory domains of Ca2+ binding proteins.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

January 25, 1985

Volume

260

Issue

2

Start / End Page

907 / 912

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Nucleic Acid Hybridization
  • Nucleic Acid Conformation
  • DNA Restriction Enzymes
  • DNA
  • Chickens
  • Calmodulin
  • Biochemistry & Molecular Biology
  • Base Sequence
  • Animals
 

Citation

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Simmen, R. C., Tanaka, T., Ts’ui, K. F., Putkey, J. A., Scott, M. J., Lai, E. C., & Means, A. R. (1985). The structural organization of the chicken calmodulin gene. J Biol Chem, 260(2), 907–912.
Simmen, R. C., T. Tanaka, K. F. Ts’ui, J. A. Putkey, M. J. Scott, E. C. Lai, and A. R. Means. “The structural organization of the chicken calmodulin gene.J Biol Chem 260, no. 2 (January 25, 1985): 907–12.
Simmen RC, Tanaka T, Ts’ui KF, Putkey JA, Scott MJ, Lai EC, et al. The structural organization of the chicken calmodulin gene. J Biol Chem. 1985 Jan 25;260(2):907–12.
Simmen, R. C., et al. “The structural organization of the chicken calmodulin gene.J Biol Chem, vol. 260, no. 2, Jan. 1985, pp. 907–12.
Simmen RC, Tanaka T, Ts’ui KF, Putkey JA, Scott MJ, Lai EC, Means AR. The structural organization of the chicken calmodulin gene. J Biol Chem. 1985 Jan 25;260(2):907–912.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

January 25, 1985

Volume

260

Issue

2

Start / End Page

907 / 912

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Nucleic Acid Hybridization
  • Nucleic Acid Conformation
  • DNA Restriction Enzymes
  • DNA
  • Chickens
  • Calmodulin
  • Biochemistry & Molecular Biology
  • Base Sequence
  • Animals