Female fertility is reduced in mice lacking Ca2+/calmodulin-dependent protein kinase IV.

Journal Article (Journal Article)

Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) is a serine/threonine protein kinase with limited tissue distribution. CaMKIV is highly expressed in the testis, where it is found in transcriptionally inactive elongating spermatids. We have recently generated mice deficient in CaMKIV. In the absence of CaMKIV, the exchange of sperm nuclear basic proteins in male spermatids is impaired, resulting in male infertility secondary to defective spermiogenesis. The involvement of CaMKIV in female fertility has not been addressed. Here we report that female fertility is markedly reduced in CaMKIV-deficient mice due to impaired follicular development and ovulation. CaMKIV is expressed in the ovary, where it is localized in granulosa cells. We further find that in cultured granulosa cells, CaMKIV expression and subcellular localization are hormonally regulated. As granulosa cells differentiate, CaMKIV levels decrease and the kinase translocates from the nucleus into the cytoplasm. Our results demonstrate a critical role for CaMKIV in female reproduction and point to a potential function in granulosa cell differentiation.

Full Text

Duke Authors

Cited Authors

  • Wu, JY; Gonzalez-Robayna, IJ; Richards, JS; Means, AR

Published Date

  • December 2000

Published In

Volume / Issue

  • 141 / 12

Start / End Page

  • 4777 - 4783

PubMed ID

  • 11108293

International Standard Serial Number (ISSN)

  • 0013-7227

Digital Object Identifier (DOI)

  • 10.1210/endo.141.12.7826


  • eng

Conference Location

  • United States