The calmodulin-dependent protein phosphatase catalytic subunit (calcineurin A) is an essential gene in Aspergillus nidulans.

Journal Article (Journal Article)

The gene encoding the homologue of the catalytic subunit of the Ca2+/calmodulin-regulated protein phosphatase 2B (calcineurin A) has been isolated from Aspergillus nidulans. This gene, cnaA+, is essential in this fungal system. Analysis of growth-arrested cells following gene disruption by homologous recombination reveals that they are blocked early in the cell cycle. The cnaA+ gene encodes a 2.5 kb mRNA and the deduced protein sequence is highly homologous to the calcineurin A subunit of other species. The mRNA varies in a cell cycle-dependent manner with maximal levels found early in G1 and considerably before the G1/S boundary. As calmodulin is also essential for A. nidulans cell cycle progression and levels rise before the G1/S boundary, our data suggest that calcineurin may represent a primary target for calmodulin at this cell cycle transition point.

Full Text

Duke Authors

Cited Authors

  • Rasmussen, C; Garen, C; Brining, S; Kincaid, RL; Means, RL; Means, AR

Published Date

  • August 15, 1994

Published In

Volume / Issue

  • 13 / 16

Start / End Page

  • 3917 - 3924

PubMed ID

  • 8070419

Pubmed Central ID

  • PMC395305

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1002/j.1460-2075.1994.tb06703.x


  • eng

Conference Location

  • England