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Regulation of intracellular levels of calmodulin and tubulin in normal and transformed cells.

Publication ,  Journal Article
Chafouleas, JG; Pardue, RL; Brinkley, BR; Dedman, JR; Means, AR
Published in: Proc Natl Acad Sci U S A
February 1981

Transformation of mammalian tissue culture cells by oncogenic viruses results in a 2-fold increase in the intracellular concentration of calmodulin quantitated by radioimmunoassay. The two pairs of companion cell lines used in this study were the Swiss mouse 3T3/simian virus 40-transformed 3T3 cells and the normal rat kidney (NRK)/Rous sarcoma virus-transformed NRK cells. The increased intracellular levels of calmodulin in the transformed cells are due to a greater increase in the rate of synthesis (3-fold) relative to the change in the rate of degradation (1.4-fold). On the other hand, no increases were observed in tubulin levels as quantitated by a colchicine-binding assay. The lack of change in tubulin concentration was accounted for by a 2-fold increase in the rate of degradation that is compensated by a similar increase in the rate of synthesis. The consequence of such changes in both transformed cell types is a 2-fold increase in the calmodulin-to-tubulin protein ratio relative to that in their nontransformed counterparts.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

February 1981

Volume

78

Issue

2

Start / End Page

996 / 1000

Location

United States

Related Subject Headings

  • Tubulin
  • Sulfur Radioisotopes
  • Simian virus 40
  • Rats
  • Mice
  • Methionine
  • Kinetics
  • Kidney
  • Cells, Cultured
  • Cell Transformation, Viral
 

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Chafouleas, J. G., Pardue, R. L., Brinkley, B. R., Dedman, J. R., & Means, A. R. (1981). Regulation of intracellular levels of calmodulin and tubulin in normal and transformed cells. Proc Natl Acad Sci U S A, 78(2), 996–1000. https://doi.org/10.1073/pnas.78.2.996
Chafouleas, J. G., R. L. Pardue, B. R. Brinkley, J. R. Dedman, and A. R. Means. “Regulation of intracellular levels of calmodulin and tubulin in normal and transformed cells.Proc Natl Acad Sci U S A 78, no. 2 (February 1981): 996–1000. https://doi.org/10.1073/pnas.78.2.996.
Chafouleas JG, Pardue RL, Brinkley BR, Dedman JR, Means AR. Regulation of intracellular levels of calmodulin and tubulin in normal and transformed cells. Proc Natl Acad Sci U S A. 1981 Feb;78(2):996–1000.
Chafouleas, J. G., et al. “Regulation of intracellular levels of calmodulin and tubulin in normal and transformed cells.Proc Natl Acad Sci U S A, vol. 78, no. 2, Feb. 1981, pp. 996–1000. Pubmed, doi:10.1073/pnas.78.2.996.
Chafouleas JG, Pardue RL, Brinkley BR, Dedman JR, Means AR. Regulation of intracellular levels of calmodulin and tubulin in normal and transformed cells. Proc Natl Acad Sci U S A. 1981 Feb;78(2):996–1000.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

February 1981

Volume

78

Issue

2

Start / End Page

996 / 1000

Location

United States

Related Subject Headings

  • Tubulin
  • Sulfur Radioisotopes
  • Simian virus 40
  • Rats
  • Mice
  • Methionine
  • Kinetics
  • Kidney
  • Cells, Cultured
  • Cell Transformation, Viral