Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation.

Published

Journal Article

Calcium signaling is critical for activation of T lymphocytes and has been proposed to be transduced through multiple calmodulin target proteins. Whereas the calcineurin-NFAT signaling module is critical for all mammalian T cells, the role of calmodulin-dependent kinase IV (CaMKIV) in mouse naïve CD4+ T-cell activation remains enigmatic. We have applied lentivius-mediated RNA interference of CaMKIV to human T cells and found that knockdown of CaMKIV abrogates T-cell receptor-mediated transcription of the IL-2 gene. We demonstrate that CaMKIV directly phosphorylates Cabin1, a transcriptional corepressor for myocyte enhancer factor 2, creating a docking site for 14-3-3, which causes its nuclear export. CaMKIV-mediated nuclear export of Cabin1 is likely to account for a significant part of the requirement of CaMKIV during human T-cell activation.

Full Text

Duke Authors

Cited Authors

  • Pan, F; Means, AR; Liu, JO

Published Date

  • June 15, 2005

Published In

Volume / Issue

  • 24 / 12

Start / End Page

  • 2104 - 2113

PubMed ID

  • 15902271

Pubmed Central ID

  • 15902271

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1038/sj.emboj.7600685

Language

  • eng

Conference Location

  • England