Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation.
Journal Article (Journal Article)
Calcium signaling is critical for activation of T lymphocytes and has been proposed to be transduced through multiple calmodulin target proteins. Whereas the calcineurin-NFAT signaling module is critical for all mammalian T cells, the role of calmodulin-dependent kinase IV (CaMKIV) in mouse naïve CD4+ T-cell activation remains enigmatic. We have applied lentivius-mediated RNA interference of CaMKIV to human T cells and found that knockdown of CaMKIV abrogates T-cell receptor-mediated transcription of the IL-2 gene. We demonstrate that CaMKIV directly phosphorylates Cabin1, a transcriptional corepressor for myocyte enhancer factor 2, creating a docking site for 14-3-3, which causes its nuclear export. CaMKIV-mediated nuclear export of Cabin1 is likely to account for a significant part of the requirement of CaMKIV during human T-cell activation.
Full Text
Duke Authors
Cited Authors
- Pan, F; Means, AR; Liu, JO
Published Date
- June 15, 2005
Published In
Volume / Issue
- 24 / 12
Start / End Page
- 2104 - 2113
PubMed ID
- 15902271
Pubmed Central ID
- PMC1150881
International Standard Serial Number (ISSN)
- 0261-4189
Digital Object Identifier (DOI)
- 10.1038/sj.emboj.7600685
Language
- eng
Conference Location
- England