Cell cycle control of BRCA2.

Published

Journal Article

Identifying the conditions and kinetics of the induction of BRCA2 gene expression may implicate roles for the function of the tumor suppressor gene. In this study, expression of BRCA2 mRNA is shown to be regulated by the cell cycle and associated with proliferation in normal and tumor-derived breast epithelial cells. Cells arrested in G(0) or early G1 contained low levels of BRCA2 mRNA. After release into a proliferating state, cells produced maximum levels of BRCA2 mRNA in late G1 and the S-phase. Similar cell cycle control of BRCA2 was observed in fractions of exponentially growing cells isolated by centrifugal elutriation. Expression of BRCA2 was shown to be independent of bulk DNA synthesis. In addition, the kinetics of BRCA2 mRNA up-regulation appeared to be similar to those of BRCA1, suggesting that the two genes could be commonly controlled. These results imply that these two tumor suppressor genes are utilized during growth and may have a protective role in cellular proliferation.

Full Text

Duke Authors

Cited Authors

  • Vaughn, JP; Cirisano, FD; Huper, G; Berchuck, A; Futreal, PA; Marks, JR; Iglehart, JD

Published Date

  • October 15, 1996

Published In

Volume / Issue

  • 56 / 20

Start / End Page

  • 4590 - 4594

PubMed ID

  • 8840967

Pubmed Central ID

  • 8840967

International Standard Serial Number (ISSN)

  • 0008-5472

Language

  • eng

Conference Location

  • United States