p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry.


Journal Article

Mutation and overexpression of the p53 gene have been noted in a wide range of human cancers and are thought to play a role in malignant transformation. Previously, immunohistochemical detection of p53 has been possible only in fresh-frozen tissues. We examined p53 expression in paraffin-embedded tissues from 50 epithelial ovarian cancers and 25 primary breast cancers with a modified immunohistochemical (IHC) technique developed in this laboratory, using monoclonal antibody (MAb) PAb1801. The 75 cases were selected from a group of patients in whom the expression levels had already been assessed in a fresh-tissue IHC assay. An identical staining reactivity was observed in both formalin-fixed, paraffin-embedded tissue and fresh-frozen tissue in 48 of 50 (96%) epithelial ovarian cancers and in 23 of 25 (92%) primary breast cancers. Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors.

Full Text

Duke Authors

Cited Authors

  • Kerns, BJ; Jordan, PA; Moore, MB; Humphrey, PA; Berchuck, A; Kohler, MF; Bast, RC; Iglehart, JD; Marks, JR

Published Date

  • July 1992

Published In

Volume / Issue

  • 40 / 7

Start / End Page

  • 1047 - 1051

PubMed ID

  • 1607637

Pubmed Central ID

  • 1607637

International Standard Serial Number (ISSN)

  • 0022-1554

Digital Object Identifier (DOI)

  • 10.1177/40.7.1607637


  • eng

Conference Location

  • United States