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Comparative genomic hybridization studies in hydatidiform moles and choriocarcinoma: amplification of 7q21-q31 and loss of 8p12-p21 in choriocarcinoma.

Publication ,  Journal Article
Ahmed, MN; Kim, K; Haddad, B; Berchuck, A; Qumsiyeh, MB
Published in: Cancer Genet Cytogenet
January 1, 2000

Comparative genomic hybridization (CGH) was utilized to investigate genetic changes from archived cases of choriocarcinoma (n = 12) and hydatidiform moles (n = 7). Test DNA was extracted from paraffin-embedded tissues, amplified using total universal PCR, and co-hybridized with control DNA to normal metaphases. Comparative genomic hybridization findings showed chromosomal imbalances in 9 of 12 cases of choriocarcinoma. By contrast, all hydatidiform moles showed normal CGH profiles. Consistent findings in choriocarcinoma included deletion at 8p (5 cases) and amplification at 7q (4 cases). A tumor suppressor gene (e.g., N33) at 8p and/or a growth regulator at 7q could play a role in the initiation of choriocarcinoma and its progression. This is the first study showing specific alterations in choriocarcinomas by CGH, and illustrates the utility of this technique in elucidating genetic changes in gynecological tumors.

Duke Scholars

Published In

Cancer Genet Cytogenet

DOI

ISSN

0165-4608

Publication Date

January 1, 2000

Volume

116

Issue

1

Start / End Page

10 / 15

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Pregnancy
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Nucleic Acid Hybridization
  • Middle Aged
  • Hydatidiform Mole
  • Humans
  • Gene Deletion
  • Gene Amplification
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ahmed, M. N., Kim, K., Haddad, B., Berchuck, A., & Qumsiyeh, M. B. (2000). Comparative genomic hybridization studies in hydatidiform moles and choriocarcinoma: amplification of 7q21-q31 and loss of 8p12-p21 in choriocarcinoma. Cancer Genet Cytogenet, 116(1), 10–15. https://doi.org/10.1016/s0165-4608(99)00103-x
Ahmed, M. N., K. Kim, B. Haddad, A. Berchuck, and M. B. Qumsiyeh. “Comparative genomic hybridization studies in hydatidiform moles and choriocarcinoma: amplification of 7q21-q31 and loss of 8p12-p21 in choriocarcinoma.Cancer Genet Cytogenet 116, no. 1 (January 1, 2000): 10–15. https://doi.org/10.1016/s0165-4608(99)00103-x.
Ahmed MN, Kim K, Haddad B, Berchuck A, Qumsiyeh MB. Comparative genomic hybridization studies in hydatidiform moles and choriocarcinoma: amplification of 7q21-q31 and loss of 8p12-p21 in choriocarcinoma. Cancer Genet Cytogenet. 2000 Jan 1;116(1):10–5.
Ahmed, M. N., et al. “Comparative genomic hybridization studies in hydatidiform moles and choriocarcinoma: amplification of 7q21-q31 and loss of 8p12-p21 in choriocarcinoma.Cancer Genet Cytogenet, vol. 116, no. 1, Jan. 2000, pp. 10–15. Pubmed, doi:10.1016/s0165-4608(99)00103-x.
Ahmed MN, Kim K, Haddad B, Berchuck A, Qumsiyeh MB. Comparative genomic hybridization studies in hydatidiform moles and choriocarcinoma: amplification of 7q21-q31 and loss of 8p12-p21 in choriocarcinoma. Cancer Genet Cytogenet. 2000 Jan 1;116(1):10–15.
Journal cover image

Published In

Cancer Genet Cytogenet

DOI

ISSN

0165-4608

Publication Date

January 1, 2000

Volume

116

Issue

1

Start / End Page

10 / 15

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Pregnancy
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Nucleic Acid Hybridization
  • Middle Aged
  • Hydatidiform Mole
  • Humans
  • Gene Deletion
  • Gene Amplification