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Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial cells.

Publication ,  Journal Article
Lidor, YJ; Xu, FJ; Martínez-Maza, O; Olt, GJ; Marks, JR; Berchuck, A; Ramakrishnan, S; Berek, JS; Bast, RC
Published in: Exp Cell Res
August 1993

Normal and neoplastic epithelial cells produce growth factors that can affect cells from different lineages. Epithelial ovarian cancers produce M-CSF and IL-6. In the present study, production of these cytokines has been measured in the apparently normal epithelial cells from which epithelial ovarian neoplasms are thought to arise. Epithelial cells from the surface of premenopausal human ovaries were established in short-term cultures. The cells bound anti-cytokeratin antibodies and exhibited characteristic epithelial morphology by light and transmission electron microscopy. M-CSF and IL-6 were detected in supernatants from cultures of these cells, using assays specific for each factor. Cytokine levels were comparable to those in supernatants from ovarian and breast cancer cell lines. M-CSF expression could also be demonstrated by immunohistochemical analysis with specific rabbit heteroantiserum. Thus, M-CSF and IL-6 are produced constitutively by normal as well as by neoplastic ovarian epithelium.

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Published In

Exp Cell Res

DOI

ISSN

0014-4827

Publication Date

August 1993

Volume

207

Issue

2

Start / End Page

332 / 339

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Radioimmunoassay
  • Ovary
  • Ovarian Neoplasms
  • Microscopy, Electron
  • Macrophage Colony-Stimulating Factor
  • Interleukin-6
  • Immunohistochemistry
  • Humans
  • Female
 

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Lidor, Y. J., Xu, F. J., Martínez-Maza, O., Olt, G. J., Marks, J. R., Berchuck, A., … Bast, R. C. (1993). Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial cells. Exp Cell Res, 207(2), 332–339. https://doi.org/10.1006/excr.1993.1200
Lidor, Y. J., F. J. Xu, O. Martínez-Maza, G. J. Olt, J. R. Marks, A. Berchuck, S. Ramakrishnan, J. S. Berek, and R. C. Bast. “Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial cells.Exp Cell Res 207, no. 2 (August 1993): 332–39. https://doi.org/10.1006/excr.1993.1200.
Lidor YJ, Xu FJ, Martínez-Maza O, Olt GJ, Marks JR, Berchuck A, et al. Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial cells. Exp Cell Res. 1993 Aug;207(2):332–9.
Lidor, Y. J., et al. “Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial cells.Exp Cell Res, vol. 207, no. 2, Aug. 1993, pp. 332–39. Pubmed, doi:10.1006/excr.1993.1200.
Lidor YJ, Xu FJ, Martínez-Maza O, Olt GJ, Marks JR, Berchuck A, Ramakrishnan S, Berek JS, Bast RC. Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial cells. Exp Cell Res. 1993 Aug;207(2):332–339.
Journal cover image

Published In

Exp Cell Res

DOI

ISSN

0014-4827

Publication Date

August 1993

Volume

207

Issue

2

Start / End Page

332 / 339

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Radioimmunoassay
  • Ovary
  • Ovarian Neoplasms
  • Microscopy, Electron
  • Macrophage Colony-Stimulating Factor
  • Interleukin-6
  • Immunohistochemistry
  • Humans
  • Female