Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial cells.
Journal Article (Journal Article)
Normal and neoplastic epithelial cells produce growth factors that can affect cells from different lineages. Epithelial ovarian cancers produce M-CSF and IL-6. In the present study, production of these cytokines has been measured in the apparently normal epithelial cells from which epithelial ovarian neoplasms are thought to arise. Epithelial cells from the surface of premenopausal human ovaries were established in short-term cultures. The cells bound anti-cytokeratin antibodies and exhibited characteristic epithelial morphology by light and transmission electron microscopy. M-CSF and IL-6 were detected in supernatants from cultures of these cells, using assays specific for each factor. Cytokine levels were comparable to those in supernatants from ovarian and breast cancer cell lines. M-CSF expression could also be demonstrated by immunohistochemical analysis with specific rabbit heteroantiserum. Thus, M-CSF and IL-6 are produced constitutively by normal as well as by neoplastic ovarian epithelium.
Full Text
Duke Authors
Cited Authors
- Lidor, YJ; Xu, FJ; Martínez-Maza, O; Olt, GJ; Marks, JR; Berchuck, A; Ramakrishnan, S; Berek, JS; Bast, RC
Published Date
- August 1993
Published In
Volume / Issue
- 207 / 2
Start / End Page
- 332 - 339
PubMed ID
- 8344385
International Standard Serial Number (ISSN)
- 0014-4827
Digital Object Identifier (DOI)
- 10.1006/excr.1993.1200
Language
- eng
Conference Location
- United States