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Mutation of the p53 tumor-suppressor gene is not a feature of endometrial hyperplasias.

Publication ,  Journal Article
Kohler, MF; Nishii, H; Humphrey, PA; Saski, H; Marks, J; Bast, RC; Clarke-Pearson, DL; Boyd, J; Berchuck, A
Published in: Am J Obstet Gynecol
September 1993

OBJECTIVE: Mutation and overexpression of the p53 gene occur in approximately 20% of endometrial carcinomas. To determine whether alteration of the p53 gene is an early event in endometrial carcinogenesis, we examined the p53 gene in endometrial hyperplasias. STUDY DESIGN: Genomic deoxyribonucleic acid was extracted from 117 endometrial hyperplasias (36 simple, 40 complex, 41 atypical) and 30 endometrial cancers. Exons 5 through 8 of the p53 gene were amplified by means of the polymerase chain reaction. Mutations in the p53 gene were sought with single-stranded conformation polymorphism analysis and confirmed by direct deoxyribonucleic acid sequencing. RESULTS: None of 117 endometrial hyperplasias were found to have mutations in the p53 gene, whereas mutations were seen in three of 30 (10%) endometrial cancers (p < 0.02). The p53 mutations seen in three cancers were confirmed by direct sequencing (codons 157, 180, 272). CONCLUSION: Because it does not appear to be a feature of endometrial hyperplasias, mutation of the p53 gene may represent a relatively late event in endometrial carcinogenesis.

Duke Scholars

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

September 1993

Volume

169

Issue

3

Start / End Page

690 / 694

Location

United States

Related Subject Headings

  • Polymorphism, Genetic
  • Polymerase Chain Reaction
  • Oligodeoxyribonucleotides
  • Obstetrics & Reproductive Medicine
  • Mutation
  • Molecular Sequence Data
  • Molecular Conformation
  • Humans
  • Genes, p53
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
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Kohler, M. F., Nishii, H., Humphrey, P. A., Saski, H., Marks, J., Bast, R. C., … Berchuck, A. (1993). Mutation of the p53 tumor-suppressor gene is not a feature of endometrial hyperplasias. Am J Obstet Gynecol, 169(3), 690–694. https://doi.org/10.1016/0002-9378(93)90644-x
Kohler, M. F., H. Nishii, P. A. Humphrey, H. Saski, J. Marks, R. C. Bast, D. L. Clarke-Pearson, J. Boyd, and A. Berchuck. “Mutation of the p53 tumor-suppressor gene is not a feature of endometrial hyperplasias.Am J Obstet Gynecol 169, no. 3 (September 1993): 690–94. https://doi.org/10.1016/0002-9378(93)90644-x.
Kohler MF, Nishii H, Humphrey PA, Saski H, Marks J, Bast RC, et al. Mutation of the p53 tumor-suppressor gene is not a feature of endometrial hyperplasias. Am J Obstet Gynecol. 1993 Sep;169(3):690–4.
Kohler, M. F., et al. “Mutation of the p53 tumor-suppressor gene is not a feature of endometrial hyperplasias.Am J Obstet Gynecol, vol. 169, no. 3, Sept. 1993, pp. 690–94. Pubmed, doi:10.1016/0002-9378(93)90644-x.
Kohler MF, Nishii H, Humphrey PA, Saski H, Marks J, Bast RC, Clarke-Pearson DL, Boyd J, Berchuck A. Mutation of the p53 tumor-suppressor gene is not a feature of endometrial hyperplasias. Am J Obstet Gynecol. 1993 Sep;169(3):690–694.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

September 1993

Volume

169

Issue

3

Start / End Page

690 / 694

Location

United States

Related Subject Headings

  • Polymorphism, Genetic
  • Polymerase Chain Reaction
  • Oligodeoxyribonucleotides
  • Obstetrics & Reproductive Medicine
  • Mutation
  • Molecular Sequence Data
  • Molecular Conformation
  • Humans
  • Genes, p53
  • Female