Mutations of the Ki-ras oncogene in endometrial carcinoma.

Journal Article (Journal Article)

OBJECTIVE: The purpose of this study was to assess the extent of involvement of the ras oncogene in endometrial carcinoma. STUDY DESIGN: Genomic deoxyribonucleic acid from 30 samples of endometrial carcinoma was examined for point mutations in codons 12, 13, and 61 from the Ha-ras, Ki-ras, and N-ras genes by means of the polymerase chain reaction, slot-blotting, and deoxyribonucleic acid sequencing procedures. RESULTS: An apparent somatic mutation of Ki-ras codon 12 in one of 10 paraffin-embedded tumors was confirmed by deoxyribonucleic acid sequence analysis. Two of 20 frozen endometrial carcinoma specimens were also shown to contain a point mutation in Ki-ras codon 12. No correlation between ras mutation and a number of histologic or clinical parameters was observed. CONCLUSIONS: These data suggest a potential role for Ki-ras codon 12 mutations in the development of some (10%) endometrial cancers.

Full Text

Duke Authors

Cited Authors

  • Ignar-Trowbridge, D; Risinger, JI; Dent, GA; Kohler, M; Berchuck, A; McLachlan, JA; Boyd, J

Published Date

  • July 1992

Published In

Volume / Issue

  • 167 / 1

Start / End Page

  • 227 - 232

PubMed ID

  • 1442931

International Standard Serial Number (ISSN)

  • 0002-9378

Digital Object Identifier (DOI)

  • 10.1016/s0002-9378(11)91663-9


  • eng

Conference Location

  • United States