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Mutation of the PTEN tumor suppressor gene in endometrial hyperplasias.

Publication ,  Journal Article
Maxwell, GL; Risinger, JI; Gumbs, C; Shaw, H; Bentley, RC; Barrett, JC; Berchuck, A; Futreal, PA
Published in: Cancer Res
June 15, 1998

Mutation and deletion of the PTEN tumor suppressor gene occurs in about 40% of endometrial carcinomas. The purpose of this study was to determine whether PTEN mutations also are present in endometrial hyperplasias, which are premalignant precursors of invasive endometrial adenocarcinomas. Genomic DNA from 51 endometrial hyperplasias was extracted from paraffin blocks, and PCR was used to amplify the nine exons of the PTEN gene. These products were screened using single-strand conformation analysis, and variant bands were sequenced. Somatic mutations in the PTEN gene were seen in 10 of 51 cases (20%), and two mutations were found in one case. An identical 4-bp deletion in exon 8 was seen in three cases, and 8 of 11 PTEN mutations predicted truncated protein products. There was no higher frequency of PTEN mutations in endometrial hyperplasias with atypia (6 of 32; 19%) relative to those without atypia (4 of 19; 21%). These data suggest that inactivation of the PTEN tumor suppressor gene is an early event in the development of some endometrial cancers.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

June 15, 1998

Volume

58

Issue

12

Start / End Page

2500 / 2503

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Protein Tyrosine Phosphatases
  • Polymerase Chain Reaction
  • Phosphoric Monoester Hydrolases
  • PTEN Phosphohydrolase
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Mutation
  • Humans
  • Genes, Tumor Suppressor
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Maxwell, G. L., Risinger, J. I., Gumbs, C., Shaw, H., Bentley, R. C., Barrett, J. C., … Futreal, P. A. (1998). Mutation of the PTEN tumor suppressor gene in endometrial hyperplasias. Cancer Res, 58(12), 2500–2503.
Maxwell, G. L., J. I. Risinger, C. Gumbs, H. Shaw, R. C. Bentley, J. C. Barrett, A. Berchuck, and P. A. Futreal. “Mutation of the PTEN tumor suppressor gene in endometrial hyperplasias.Cancer Res 58, no. 12 (June 15, 1998): 2500–2503.
Maxwell GL, Risinger JI, Gumbs C, Shaw H, Bentley RC, Barrett JC, et al. Mutation of the PTEN tumor suppressor gene in endometrial hyperplasias. Cancer Res. 1998 Jun 15;58(12):2500–3.
Maxwell, G. L., et al. “Mutation of the PTEN tumor suppressor gene in endometrial hyperplasias.Cancer Res, vol. 58, no. 12, June 1998, pp. 2500–03.
Maxwell GL, Risinger JI, Gumbs C, Shaw H, Bentley RC, Barrett JC, Berchuck A, Futreal PA. Mutation of the PTEN tumor suppressor gene in endometrial hyperplasias. Cancer Res. 1998 Jun 15;58(12):2500–2503.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

June 15, 1998

Volume

58

Issue

12

Start / End Page

2500 / 2503

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Protein Tyrosine Phosphatases
  • Polymerase Chain Reaction
  • Phosphoric Monoester Hydrolases
  • PTEN Phosphohydrolase
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Mutation
  • Humans
  • Genes, Tumor Suppressor