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Galectin-1 accumulation in the ovary carcinoma peritumoral stroma is induced by ovary carcinoma cells and affects both cancer cell proliferation and adhesion to laminin-1 and fibronectin.

Publication ,  Journal Article
van den Brûle, F; Califice, S; Garnier, F; Fernandez, PL; Berchuck, A; Castronovo, V
Published in: Lab Invest
March 2003

Galectin-1 (gal-1) is a 14-kDa laminin-binding galectin involved in several biologic events including regulation of cancer cell proliferation and adhesion to the matrix. In this study, we examined gal-1 expression in 30 human epithelial ovary carcinoma samples by Western and Northern blotting and by immunohistochemistry. Gal-1 mRNA levels were increased in more than 95% of the examined ovary carcinoma samples, compared with a wedge resection of a normal ovary. Immunohistochemical analysis of the samples demonstrated gal-1 expression in cancer epithelial cells from 17 of 30 samples, with a cytoplasmic pattern. Gal-1 immunostaining was significantly increased in the stroma associated with carcinoma cells compared with the normal, noninvaded stroma (p = 0.003). This pattern of expression was confirmed by examination of 12 other frozen epithelial ovary carcinomas, using in situ hybridization. Immunohistochemical staining of the specimens demonstrated colocalization of gal-1, laminin-1, and fibronectin. In vitro experiments were conducted to elucidate the potential biologic role of gal-1 in ovarian cancer progression. Gal-1 protein expression and release was detected in AZ364, SK-OV-3, and AZ224, but not in OVCAR-3, AZ419, and AZ382, human ovary carcinoma cell lines. Incubation of 84BR fibroblasts with conditioned media harvested from the ovary carcinoma cell lines induced an increased expression of gal-1 in the cultured fibroblasts in all cases except AZ419 and SK-OV-3. High concentrations of gal-1 (100 micro g/ml) induced significantly decreased cell proliferation in all cell lines, as defined by bromodeoxyuridine incorporation. Additionally, recombinant gal-1 induced a dose-dependent increase in in vitro adhesion of AZ224, SK-OV-3, and AZ382 cells to laminin-1; adhesion to fibronectin was increased by gal-1 in OVCAR-3, AZ224, and SK-OV-3. No effect was observed in the other cases. Our data contribute to define a role for gal-1 during the interactions between human ovary carcinoma cells and host fibroblasts.

Duke Scholars

Published In

Lab Invest

DOI

ISSN

0023-6837

Publication Date

March 2003

Volume

83

Issue

3

Start / End Page

377 / 386

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Stromal Cells
  • RNA, Messenger
  • Pathology
  • Ovarian Neoplasms
  • Neoplasm Staging
  • Laminin
  • In Situ Hybridization
  • Immunoenzyme Techniques
  • Humans
 

Citation

APA
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ICMJE
MLA
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van den Brûle, F., Califice, S., Garnier, F., Fernandez, P. L., Berchuck, A., & Castronovo, V. (2003). Galectin-1 accumulation in the ovary carcinoma peritumoral stroma is induced by ovary carcinoma cells and affects both cancer cell proliferation and adhesion to laminin-1 and fibronectin. Lab Invest, 83(3), 377–386. https://doi.org/10.1097/01.lab.0000059949.01480.40
Brûle, Frédéric van den, Stéphane Califice, Frédérique Garnier, Pedro L. Fernandez, Andrew Berchuck, and Vincent Castronovo. “Galectin-1 accumulation in the ovary carcinoma peritumoral stroma is induced by ovary carcinoma cells and affects both cancer cell proliferation and adhesion to laminin-1 and fibronectin.Lab Invest 83, no. 3 (March 2003): 377–86. https://doi.org/10.1097/01.lab.0000059949.01480.40.
van den Brûle, Frédéric, et al. “Galectin-1 accumulation in the ovary carcinoma peritumoral stroma is induced by ovary carcinoma cells and affects both cancer cell proliferation and adhesion to laminin-1 and fibronectin.Lab Invest, vol. 83, no. 3, Mar. 2003, pp. 377–86. Pubmed, doi:10.1097/01.lab.0000059949.01480.40.

Published In

Lab Invest

DOI

ISSN

0023-6837

Publication Date

March 2003

Volume

83

Issue

3

Start / End Page

377 / 386

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Stromal Cells
  • RNA, Messenger
  • Pathology
  • Ovarian Neoplasms
  • Neoplasm Staging
  • Laminin
  • In Situ Hybridization
  • Immunoenzyme Techniques
  • Humans