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Mutational analysis of the PTEN gene in human uterine sarcomas.

Publication ,  Journal Article
Lancaster, JM; Risinger, JI; Carney, ME; Barrett, JC; Berchuck, A
Published in: Am J Obstet Gynecol
May 2001

OBJECTIVE: Uterine sarcomas are rare, lethal cancers, and little is known about their molecular etiology. The PTEN gene is located on chromosome 10q23.3, a region that displays frequent loss of heterozygosity in human uterine sarcomas. PTEN mutations have been described in 40% to 60% of uterine adenocarcinomas. To determine whether the PTEN gene is involved in the pathogenesis of uterine sarcoma, we analyzed deoxyribonucleic acid from uterine sarcomas and cell lines. STUDY DESIGN: Single-strand conformation analysis and direct sequencing of deoxyribonucleic acid were used to screen for PTEN mutations. RESULTS: Silent polymorphisms were detected in 2 of 36 primary uterine sarcomas. A 4-base pair deletion and a point mutation producing a stop codon were identified in 1 cell line. CONCLUSIONS: Mutational inactivation of PTEN does not play a major role in uterine sarcoma tumorigenesis, and another gene or genes on chromosome 10q may be implicated as a cause of these cancers. Differences in the molecular alterations underlying the development of uterine sarcomas and adenocarcinomas are significant.

Duke Scholars

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

May 2001

Volume

184

Issue

6

Start / End Page

1051 / 1053

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Tumor Suppressor Proteins
  • Tumor Cells, Cultured
  • Sarcoma
  • Polymorphism, Genetic
  • Point Mutation
  • Phosphoric Monoester Hydrolases
  • PTEN Phosphohydrolase
  • Obstetrics & Reproductive Medicine
  • Humans
 

Citation

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MLA
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Lancaster, J. M., Risinger, J. I., Carney, M. E., Barrett, J. C., & Berchuck, A. (2001). Mutational analysis of the PTEN gene in human uterine sarcomas. Am J Obstet Gynecol, 184(6), 1051–1053. https://doi.org/10.1067/mob.2001.114508
Lancaster, J. M., J. I. Risinger, M. E. Carney, J. C. Barrett, and A. Berchuck. “Mutational analysis of the PTEN gene in human uterine sarcomas.Am J Obstet Gynecol 184, no. 6 (May 2001): 1051–53. https://doi.org/10.1067/mob.2001.114508.
Lancaster JM, Risinger JI, Carney ME, Barrett JC, Berchuck A. Mutational analysis of the PTEN gene in human uterine sarcomas. Am J Obstet Gynecol. 2001 May;184(6):1051–3.
Lancaster, J. M., et al. “Mutational analysis of the PTEN gene in human uterine sarcomas.Am J Obstet Gynecol, vol. 184, no. 6, May 2001, pp. 1051–53. Pubmed, doi:10.1067/mob.2001.114508.
Lancaster JM, Risinger JI, Carney ME, Barrett JC, Berchuck A. Mutational analysis of the PTEN gene in human uterine sarcomas. Am J Obstet Gynecol. 2001 May;184(6):1051–1053.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

May 2001

Volume

184

Issue

6

Start / End Page

1051 / 1053

Location

United States

Related Subject Headings

  • Uterine Neoplasms
  • Tumor Suppressor Proteins
  • Tumor Cells, Cultured
  • Sarcoma
  • Polymorphism, Genetic
  • Point Mutation
  • Phosphoric Monoester Hydrolases
  • PTEN Phosphohydrolase
  • Obstetrics & Reproductive Medicine
  • Humans