Recognition by and in vitro induction of cytotoxic T lymphocytes against predicted epitopes of the immediate-early protein ICP27 of herpes simplex virus.
Journal Article (Journal Article)
The identification of herpes simplex virus type 1 (HSV-1) proteins and the minimal epitopes within these proteins which serve as targets for cytotoxic T lymphocytes (CTL) remains an important goal for the development of effective vaccine strategies. In this report, an H-2Kd allele-specific peptide-binding motif was used to locate putative CTL epitopes in the HSV-1 immediate-early protein ICP27, a protein previously identified as a major CTL target in the BALB/c mouse. Peptides 1 (amino acids 322 to 332) and 2 (amino acids 448 to 456) synthesized to represent two separate predicted CTL epitopes in ICP27 were able to sensitize target cells in vitro for recognition by HSV-1-specific CTL. Moreover, using a recently developed system to generate primary CTL responses in vitro, both peptides induced primary CTL which reacted with target cells expressing HSV-1. This system allowed us to verify the activity of two CTL epitopes in the ICP27 protein and holds promise as a rapid way of identifying immunogenic peptides from any protein molecule.
Full Text
Duke Authors
Cited Authors
- Banks, TA; Nair, S; Rouse, BT
Published Date
- January 1, 1993
Published In
Volume / Issue
- 67 / 1
Start / End Page
- 613 - 616
PubMed ID
- 7677961
Pubmed Central ID
- PMC237406
International Standard Serial Number (ISSN)
- 0022-538X
Digital Object Identifier (DOI)
- 10.1128/JVI.67.1.613-616.1993
Language
- eng
Conference Location
- United States