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Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors.

Publication ,  Journal Article
Ashley, DM; Faiola, B; Nair, S; Hale, LP; Bigner, DD; Gilboa, E
Published in: J Exp Med
October 6, 1997

Recent studies have shown that the brain is not a barrier to successful active immunotherapy that uses gene-modified autologous tumor cell vaccines. In this study, we compared the efficacy of two types of vaccines for the treatment of tumors within the central nervous system (CNS): dendritic cell (DC)-based vaccines pulsed with either tumor extract or tumor RNA, and cytokine gene-modified tumor vaccines. Using the B16/F10 murine melanoma (B16) as a model for CNS tumor, we show that vaccination with bone marrow-generated DCs, pulsed with either B16 cell extract or B16 total RNA, can induce specific cytotoxic T lymphocytes against B16 tumor cells. Both types of DC vaccines were able to protect animals from tumors located in the CNS. DC-based vaccines also led to prolonged survival in mice with tumors placed before the initiation of vaccine therapy. The DC-based vaccines were at least as effective, if not more so, as vaccines containing B16 tumor cells in which the granulocytic macrophage colony-stimulating factor gene had been modified. These data support the use of DC-based vaccines for the treatment of patients with CNS tumors.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

October 6, 1997

Volume

186

Issue

7

Start / End Page

1177 / 1182

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • RNA, Neoplasm
  • Mice
  • Melanoma, Experimental
  • Immunotherapy
  • Immunology
  • Histocytochemistry
  • Dendritic Cells
  • Central Nervous System Neoplasms
 

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Ashley, D. M., Faiola, B., Nair, S., Hale, L. P., Bigner, D. D., & Gilboa, E. (1997). Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors. J Exp Med, 186(7), 1177–1182. https://doi.org/10.1084/jem.186.7.1177
Ashley, D. M., B. Faiola, S. Nair, L. P. Hale, D. D. Bigner, and E. Gilboa. “Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors.J Exp Med 186, no. 7 (October 6, 1997): 1177–82. https://doi.org/10.1084/jem.186.7.1177.
Ashley DM, Faiola B, Nair S, Hale LP, Bigner DD, Gilboa E. Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors. J Exp Med. 1997 Oct 6;186(7):1177–82.
Ashley, D. M., et al. “Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors.J Exp Med, vol. 186, no. 7, Oct. 1997, pp. 1177–82. Pubmed, doi:10.1084/jem.186.7.1177.
Ashley DM, Faiola B, Nair S, Hale LP, Bigner DD, Gilboa E. Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors. J Exp Med. 1997 Oct 6;186(7):1177–1182.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

October 6, 1997

Volume

186

Issue

7

Start / End Page

1177 / 1182

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • RNA, Neoplasm
  • Mice
  • Melanoma, Experimental
  • Immunotherapy
  • Immunology
  • Histocytochemistry
  • Dendritic Cells
  • Central Nervous System Neoplasms