Class I restricted CTL recognition of a soluble protein delivered by liposomes containing lipophilic polylysines.
Journal Article (Journal Article)
CD8+ cytotoxic lymphocytes recognize peptides derived from endogenous antigens complexed with class I major histocompatibility complex while CD4+ helper cells recognize peptides from exogenous antigens bound to class II MHC molecules. A soluble protein can be introduced into the class I pathway of antigen processing and presentation using an appropriate vehicle to deliver the antigen into the cytosol. Cationic liposomes containing lipophilic polylysine readily form complexes with an anionic, soluble protein ovalbumin. Mouse thymoma EL4 cells incubated with such complexes can be sensitized for killing by OVA-specific CTL effector cells. This method of target sensitization by a soluble antigen is more sensitive than the osmotic loading method previously reported.
Full Text
Duke Authors
Cited Authors
- Nair, S; Zhou, X; Huang, L; Rouse, BT
Published Date
- August 10, 1992
Published In
Volume / Issue
- 152 / 2
Start / End Page
- 237 - 243
PubMed ID
- 1386866
International Standard Serial Number (ISSN)
- 0022-1759
Digital Object Identifier (DOI)
- 10.1016/0022-1759(92)90145-j
Language
- eng
Conference Location
- Netherlands