Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells.

Journal Article

Dendritic cells (DCs), matured by CD40-ligand (CD40L), undergo marked changes in their ability to process and present antigen, resulting in augmented lymphocyte stimulatory activity. We demonstrate that the form of the tumor antigen (peptide or genetic material) used to load the DCs dictates the required sequence of antigen loading and maturation for antitumor immunotherapy. Optimal stimulation of carcinoembryonic antigen (CEA)-specific CTLs by peptide-loaded DCs occurs when DCs from cancer patients are matured with CD40L before exposure to CEA peptide, whereas optimal stimulation by RNA-transfected DCs occurs when the DCs are loaded with CEA RNA before maturation with CD40L.

Full Text

Duke Authors

Cited Authors

  • Morse, MA; Lyerly, HK; Gilboa, E; Thomas, E; Nair, SK

Published Date

  • July 15, 1998

Published In

Volume / Issue

  • 58 / 14

Start / End Page

  • 2965 - 2968

PubMed ID

  • 9679955

International Standard Serial Number (ISSN)

  • 0008-5472

Language

  • eng

Conference Location

  • United States