The immunohistochemical profile of the myelomeningocele placode: is the placode normal?

Published

Journal Article

One key to predicting optimal outcome of novel treatments, such as fetal surgery, for myelomeningocele is understanding the structure of the placode. We hypothesize that if the placode retains normal patterning and is simply unneurulated, then repair may be effective in preventing secondary injury. Serial sections of human myelomeningocele placodes were characterized using novel immunohistochemical caudal spinal cord markers (H4C4, AC4, NOT, VIN-IS-53, and FP3) and established structural markers (e.g. neurofilament, glial fibrillary acidic protein, or synaptophysin). The myelomeningocele placodes studied exhibited abnormal patterning along the dorsoventral and rostrocaudal axes indicative of a change in pattern determination and a paucity of maturing neurons. In addition, there was significant inflammatory infiltrate, gliosis and fibrosis consistent with secondary injury. Additionally, injury to the placode was also demonstrated. The combination of effects may be contributory to abnormal spinal cord function in myelomeningocele patients.

Full Text

Duke Authors

Cited Authors

  • George, TM; Cummings, TJ

Published Date

  • November 2003

Published In

Volume / Issue

  • 39 / 5

Start / End Page

  • 234 - 239

PubMed ID

  • 14512686

Pubmed Central ID

  • 14512686

International Standard Serial Number (ISSN)

  • 1016-2291

Digital Object Identifier (DOI)

  • 10.1159/000072867

Language

  • eng

Conference Location

  • Switzerland