Long-term behavioral assessment of guinea pigs following neonatal pneumoperitoneum.
Journal Article
BACKGROUND: Using guinea pigs, we previously demonstrated that pneumoperitoneum during pregnancy produces behavioral deficits in the offspring. In the current study, the purpose was to determine if CO(2) pneumoperitoneum during the early postnatal period also produced behavioral anomalies. METHODS: Following delivery, guinea pig pups were randomly assigned to one of three treatment groups: CO(2) pneumoperitoneum (P), laparotomy (L), or isolation control (I). Surgeries were performed on postnatal day (PND) 5 under isoflurane anesthesia; control pups were isolated from the dams for an equivalent period of time. On PNDs 10, 20, 40, and 60, behavior was assessed by monitoring locomotor and exploratory activity. RESULTS: A total of 29 animals were studied. We observed no immediate morbidity or mortality and the manipulations did not appear to affect postnatal growth. On PND 10, pups in group P exhibited lower levels of locomotor activity compared to L and I neonates, but this difference resolved as the animals got older. Histologic assessment of the adult offspring brains revealed no evidence of neurologic injury. CONCLUSION: These data suggest that unlike insufflation during pregnancy, neonatal pneumoperitoneum does not produce behavioral deficits.
Full Text
Duke Authors
Cited Authors
- Fuh, E; de la Fuente, S; Shah, MK; Okodiko, DK; Cummings, TJ; Eubanks, WS; Reynolds, JD
Published Date
- May 2005
Published In
Volume / Issue
- 19 / 5
Start / End Page
- 715 - 719
PubMed ID
- 15759194
Pubmed Central ID
- 15759194
Electronic International Standard Serial Number (EISSN)
- 1432-2218
Digital Object Identifier (DOI)
- 10.1007/s00464-004-8181-x
Language
- eng
Conference Location
- Germany