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The MTE, a new core promoter element for transcription by RNA polymerase II.

Publication ,  Journal Article
Lim, CY; Santoso, B; Boulay, T; Dong, E; Ohler, U; Kadonaga, JT
Published in: Genes Dev
July 1, 2004

The core promoter is the ultimate target of the vast network of regulatory factors that contribute to the initiation of transcription by RNA polymerase II. Here we describe the MTE (motif ten element), a new core promoter element that appears to be conserved from Drosophila to humans. The MTE promotes transcription by RNA polymerase II when it is located precisely at positions +18 to +27 relative to A(+1) in the initiator (Inr) element. MTE sequences from +18 to +22 relative to A(+1) are important for basal transcription, and a region from +18 to +27 is sufficient to confer MTE activity to heterologous core promoters. The MTE requires the Inr, but functions independently of the TATA-box and DPE. Notably, the loss of transcriptional activity upon mutation of a TATA-box or DPE can be compensated by the addition of an MTE. In addition, the MTE exhibits strong synergism with the TATA-box as well as the DPE. These findings indicate that the MTE is a novel downstream core promoter element that is important for transcription by RNA polymerase II.

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Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

July 1, 2004

Volume

18

Issue

13

Start / End Page

1606 / 1617

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Transcription Factor TFIID
  • TATA Box
  • RNA Polymerase II
  • Promoter Regions, Genetic
  • Humans
  • Hela Cells
  • HeLa Cells
  • Developmental Biology
 

Citation

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Lim, C. Y., Santoso, B., Boulay, T., Dong, E., Ohler, U., & Kadonaga, J. T. (2004). The MTE, a new core promoter element for transcription by RNA polymerase II. Genes Dev, 18(13), 1606–1617. https://doi.org/10.1101/gad.1193404
Lim, Chin Yan, Buyung Santoso, Thomas Boulay, Emily Dong, Uwe Ohler, and James T. Kadonaga. “The MTE, a new core promoter element for transcription by RNA polymerase II.Genes Dev 18, no. 13 (July 1, 2004): 1606–17. https://doi.org/10.1101/gad.1193404.
Lim CY, Santoso B, Boulay T, Dong E, Ohler U, Kadonaga JT. The MTE, a new core promoter element for transcription by RNA polymerase II. Genes Dev. 2004 Jul 1;18(13):1606–17.
Lim, Chin Yan, et al. “The MTE, a new core promoter element for transcription by RNA polymerase II.Genes Dev, vol. 18, no. 13, July 2004, pp. 1606–17. Pubmed, doi:10.1101/gad.1193404.
Lim CY, Santoso B, Boulay T, Dong E, Ohler U, Kadonaga JT. The MTE, a new core promoter element for transcription by RNA polymerase II. Genes Dev. 2004 Jul 1;18(13):1606–1617.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

July 1, 2004

Volume

18

Issue

13

Start / End Page

1606 / 1617

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Transcription Factor TFIID
  • TATA Box
  • RNA Polymerase II
  • Promoter Regions, Genetic
  • Humans
  • Hela Cells
  • HeLa Cells
  • Developmental Biology