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Relationship of DNA ploidy to histology and prognosis in rhabdomyosarcoma. Comparison of flow cytometry and image analysis.

Publication ,  Journal Article
Kilpatrick, SE; Teot, LA; Geisinger, KR; Martin, PL; Shumate, DK; Zbieranski, N; Russell, GB; Fletcher, CD
Published in: Cancer
December 15, 1994

BACKGROUND: Although DNA ploidy correlates with prognosis in certain childhood cancers, e.g., neuroblastoma, its significance in rhabdomyosarcoma (RMS) is unclear and controversial. METHODS: Ploidy by flow cytometry (FCM) and image analysis (IA) in 26 of 27 children with RMS (17 embryonal, 3 mixed embryonal/alveolar, 5 alveolar, 1 anaplastic, 1 ectomesenchymoma) and 4 adults with pleomorphic RMS were evaluated. Statistical comparisons were analyzed between DNA content and gender, age, localization, Intergroup Rhabdomyosarcoma Study (IRS) group, and histopathologic subtype. Survival analyses were performed by the Kaplan-Meier test using the approximate chi-square statistic for the log rank test. RESULTS: The concordance rate between FCM and IA was 26 of 30 (87%); FCM was not performed in one tumor. Image analysis was more sensitive than FCM in detecting aneuploidy. Furthermore, DNA content was associated significantly with histologic subtype (P = 0.031); embryonal histology commonly was hyperdiploid (mean, 1.44; median, 1.27), whereas alveolar histology usually was near-tetraploid (mean, 1.83; median, 1.95). All four adult patients with pleomorphic RMS were aneuploid, with one showing multiple DNA peaks. No correlation between DNA content and survival was observed in the children with RMS. However, IRS group (P = 0.011) and patient age (P = 0.036) were independent prognostic indicators significantly related to survival. All adult patients died of their disease. CONCLUSIONS: Although ploidy correlates with histologic subtype, DNA content is not significantly predictive of prognosis in patients with RMS. Age at diagnosis and IRS group are independent predictors of clinical outcome in children with RMS.

Duke Scholars

Published In

Cancer

DOI

ISSN

0008-543X

Publication Date

December 15, 1994

Volume

74

Issue

12

Start / End Page

3227 / 3233

Location

United States

Related Subject Headings

  • Survival Analysis
  • Staining and Labeling
  • Soft Tissue Neoplasms
  • Rhabdomyosarcoma
  • Retrospective Studies
  • Prognosis
  • Ploidies
  • Oncology & Carcinogenesis
  • Male
  • Infant
 

Citation

APA
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MLA
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Kilpatrick, S. E., Teot, L. A., Geisinger, K. R., Martin, P. L., Shumate, D. K., Zbieranski, N., … Fletcher, C. D. (1994). Relationship of DNA ploidy to histology and prognosis in rhabdomyosarcoma. Comparison of flow cytometry and image analysis. Cancer, 74(12), 3227–3233. https://doi.org/10.1002/1097-0142(19941215)74:12<3227::aid-cncr2820741223>3.0.co;2-x
Kilpatrick, S. E., L. A. Teot, K. R. Geisinger, P. L. Martin, D. K. Shumate, N. Zbieranski, G. B. Russell, and C. D. Fletcher. “Relationship of DNA ploidy to histology and prognosis in rhabdomyosarcoma. Comparison of flow cytometry and image analysis.Cancer 74, no. 12 (December 15, 1994): 3227–33. https://doi.org/10.1002/1097-0142(19941215)74:12<3227::aid-cncr2820741223>3.0.co;2-x.
Kilpatrick SE, Teot LA, Geisinger KR, Martin PL, Shumate DK, Zbieranski N, et al. Relationship of DNA ploidy to histology and prognosis in rhabdomyosarcoma. Comparison of flow cytometry and image analysis. Cancer. 1994 Dec 15;74(12):3227–33.
Kilpatrick, S. E., et al. “Relationship of DNA ploidy to histology and prognosis in rhabdomyosarcoma. Comparison of flow cytometry and image analysis.Cancer, vol. 74, no. 12, Dec. 1994, pp. 3227–33. Pubmed, doi:10.1002/1097-0142(19941215)74:12<3227::aid-cncr2820741223>3.0.co;2-x.
Kilpatrick SE, Teot LA, Geisinger KR, Martin PL, Shumate DK, Zbieranski N, Russell GB, Fletcher CD. Relationship of DNA ploidy to histology and prognosis in rhabdomyosarcoma. Comparison of flow cytometry and image analysis. Cancer. 1994 Dec 15;74(12):3227–3233.
Journal cover image

Published In

Cancer

DOI

ISSN

0008-543X

Publication Date

December 15, 1994

Volume

74

Issue

12

Start / End Page

3227 / 3233

Location

United States

Related Subject Headings

  • Survival Analysis
  • Staining and Labeling
  • Soft Tissue Neoplasms
  • Rhabdomyosarcoma
  • Retrospective Studies
  • Prognosis
  • Ploidies
  • Oncology & Carcinogenesis
  • Male
  • Infant