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Evidence for platelet-activating factor secretion during immune activation in dogs.

Publication ,  Journal Article
Munoz, NM; Stimler-Gerard, NP; Mack, MM; Tutins, C; Blake, JS; Kelly, E; Murphy, TM; Leff, AR
Published in: J Appl Physiol (1985)
April 1989

To elucidate the potential physiological significance of platelet-activating factor (PAF) in acute bronchoconstriction, we studied the effect of Ascaris suum antigen on the tachyphylactic response to PAF in 15 natively allergic mongrel dogs in vivo. Active bronchial tension was measured isometrically, and mediator secretion was measured as the arteriovenous difference (AVd) in plasma concentration across the lungs. Administration of PAF into the bronchial artery caused dose-related contraction in five control dogs (maximal active tension = 11.8 +/- 1.68 g/cm) that paralleled the increase in the AVd for serotonin (4,188 +/- 175 pg/ml) but not histamine (maximal AVd less than 6.0 ng/ml). The response to PAF was highly tachyphylactic. In contrast to PAF, 1:10 concentration of intra-arterial antigen caused substantial release of histamine (AVd = 308 +/- 57.1 ng/ml; P less than 0.001 vs. PAF). Diminished responsiveness (2-log shift in threshold and maximal contraction; P less than 0.001) to PAF was demonstrated in five dogs after 1:10 antigen, compatible with endogenous release of PAF during prior immune challenge in the same animals. Administration of Ascaris antigen caused a leftward shift in the dose-response curve to serotonin and only mild tachyphylaxis to the maximal response to histamine. Our data are compatible with physiological participation of PAF in eliciting bronchial smooth muscle contraction during the acute phase of immune activation caused by A. suum antigen.

Duke Scholars

Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

April 1989

Volume

66

Issue

4

Start / End Page

1860 / 1866

Location

United States

Related Subject Headings

  • Tachyphylaxis
  • Platelet Activating Factor
  • Physiology
  • Male
  • Female
  • Dogs
  • Bronchi
  • Antibody Formation
  • Animals
  • 11 Medical and Health Sciences
 

Citation

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Munoz, N. M., Stimler-Gerard, N. P., Mack, M. M., Tutins, C., Blake, J. S., Kelly, E., … Leff, A. R. (1989). Evidence for platelet-activating factor secretion during immune activation in dogs. J Appl Physiol (1985), 66(4), 1860–1866. https://doi.org/10.1152/jappl.1989.66.4.1860
Munoz, N. M., N. P. Stimler-Gerard, M. M. Mack, C. Tutins, J. S. Blake, E. Kelly, T. M. Murphy, and A. R. Leff. “Evidence for platelet-activating factor secretion during immune activation in dogs.J Appl Physiol (1985) 66, no. 4 (April 1989): 1860–66. https://doi.org/10.1152/jappl.1989.66.4.1860.
Munoz NM, Stimler-Gerard NP, Mack MM, Tutins C, Blake JS, Kelly E, et al. Evidence for platelet-activating factor secretion during immune activation in dogs. J Appl Physiol (1985). 1989 Apr;66(4):1860–6.
Munoz, N. M., et al. “Evidence for platelet-activating factor secretion during immune activation in dogs.J Appl Physiol (1985), vol. 66, no. 4, Apr. 1989, pp. 1860–66. Pubmed, doi:10.1152/jappl.1989.66.4.1860.
Munoz NM, Stimler-Gerard NP, Mack MM, Tutins C, Blake JS, Kelly E, Murphy TM, Leff AR. Evidence for platelet-activating factor secretion during immune activation in dogs. J Appl Physiol (1985). 1989 Apr;66(4):1860–1866.

Published In

J Appl Physiol (1985)

DOI

ISSN

8750-7587

Publication Date

April 1989

Volume

66

Issue

4

Start / End Page

1860 / 1866

Location

United States

Related Subject Headings

  • Tachyphylaxis
  • Platelet Activating Factor
  • Physiology
  • Male
  • Female
  • Dogs
  • Bronchi
  • Antibody Formation
  • Animals
  • 11 Medical and Health Sciences