High-level synthesis of biologically active reovirus protein sigma 1 in a mammalian expression vector system.

Published

Journal Article

The reovirus cell-attachment protein, which modulates tissue tropism and the nature of the antiviral immune response, is protein sigma 1. This protein is present in reovirus particles in the form of 12 tetramers anchored in the projections or spikes. It is formed in infected cells in very small amounts only. In order to produce larger amounts of it that are necessary for studying its properties in detail, we have cloned it into a mammalian expression vector system that consists of the very strong cowpox virus A-type inclusion body protein gene promoter inserted into the vaccinia virus genome. In cells infected with the resultant recombinant vaccina virus, protein sigma 1 is formed in large amounts (2-3 mg/10(9) cells). We have isolated the native protein and shown that it exists as tetramers that possess cell-binding activity, hemagglutinating activity, and functional epitopes recognized by both polyvalent antisera and monoclonal antibodies.

Full Text

Duke Authors

Cited Authors

  • Banerjea, AC; Brechling, KA; Ray, CA; Erikson, H; Pickup, DJ; Joklik, WK

Published Date

  • December 1988

Published In

Volume / Issue

  • 167 / 2

Start / End Page

  • 601 - 612

PubMed ID

  • 3201754

Pubmed Central ID

  • 3201754

International Standard Serial Number (ISSN)

  • 0042-6822

Language

  • eng

Conference Location

  • United States