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Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding protein.

Publication ,  Journal Article
Spriggs, MK; Hruby, DE; Maliszewski, CR; Pickup, DJ; Sims, JE; Buller, RM; VanSlyke, J
Published in: Cell
October 2, 1992

Supernatants from vaccinia virus (VV)-infected CV-1 cells were examined and found to contain a 33 kd protein capable of binding murine interleukin-1 beta (mIL-1 beta). A VV open reading frame (ORF) that exhibits 30% amino acid identity to the type II IL-1 receptor was expressed in CV-1-EBNA cells and shown specifically to bind mIL-1 beta. A similar ORF from cowpox virus was expressed and also specifically bound mIL-1 beta. A recombinant VV was constructed in which this ORF was disrupted (vB15RKO). Supernatants from vB15RKO-infected cells did not contain an IL-1-binding protein. Supernatants from VV-infected CV-1 cells were capable of inhibiting IL-1-induced murine lymphocyte proliferation in vitro while supernatants from vB15RKO infected cells did not. Intracranial inoculation of mice with vB15RKO suggests that this ORF is involved in VV virulence. The possible role of a virus-encoded IL-1-binding protein in the pathology of a poxvirus infection and its relationship to other poxvirus-encoded immune modulators is discussed.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 2, 1992

Volume

71

Issue

1

Start / End Page

145 / 152

Location

United States

Related Subject Headings

  • Viral Proteins
  • Vaccinia virus
  • Receptors, Interleukin-1
  • Plasmids
  • Open Reading Frames
  • Molecular Sequence Data
  • Lymphocyte Activation
  • Interleukin-1
  • Developmental Biology
  • Culture Media
 

Citation

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Spriggs, M. K., Hruby, D. E., Maliszewski, C. R., Pickup, D. J., Sims, J. E., Buller, R. M., & VanSlyke, J. (1992). Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding protein. Cell, 71(1), 145–152. https://doi.org/10.1016/0092-8674(92)90273-f
Spriggs, M. K., D. E. Hruby, C. R. Maliszewski, D. J. Pickup, J. E. Sims, R. M. Buller, and J. VanSlyke. “Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding protein.Cell 71, no. 1 (October 2, 1992): 145–52. https://doi.org/10.1016/0092-8674(92)90273-f.
Spriggs MK, Hruby DE, Maliszewski CR, Pickup DJ, Sims JE, Buller RM, et al. Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding protein. Cell. 1992 Oct 2;71(1):145–52.
Spriggs, M. K., et al. “Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding protein.Cell, vol. 71, no. 1, Oct. 1992, pp. 145–52. Pubmed, doi:10.1016/0092-8674(92)90273-f.
Spriggs MK, Hruby DE, Maliszewski CR, Pickup DJ, Sims JE, Buller RM, VanSlyke J. Vaccinia and cowpox viruses encode a novel secreted interleukin-1-binding protein. Cell. 1992 Oct 2;71(1):145–152.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 2, 1992

Volume

71

Issue

1

Start / End Page

145 / 152

Location

United States

Related Subject Headings

  • Viral Proteins
  • Vaccinia virus
  • Receptors, Interleukin-1
  • Plasmids
  • Open Reading Frames
  • Molecular Sequence Data
  • Lymphocyte Activation
  • Interleukin-1
  • Developmental Biology
  • Culture Media