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Discrimination of antineutrophil antibodies from antinuclear antibodies using immunofluorescence on neutrophils and HL60 cells.

Publication ,  Journal Article
Christenson, VD; Dooley, MA; Allen, NB
Published in: J Rheumatol
April 1991

Sera of 209 patients with rheumatic disease demonstrated 3 patterns of immunofluorescence (IF) on ethanol fixed neutrophils (PMN) and a promyelocytic cell line (HL60): (1) cytoplasmic on PMN, negative on HL60 cells, (2) nuclear or perinuclear on PMN, negative on HL60 and (3) nuclear or perinuclear on both PMN and HL60 cells. Pattern 1 was observed in sera of patients with Wegener's granulomatosis, but also in several patients with other rheumatic diseases. Pattern 2 was observed in sera of patients with systemic vasculitis or rheumatoid arthritis (RA). Antimyeloperoxidase activity was detected in sera from patients with systemic vasculitis that produced Pattern 2, but rarely in sera of patients with RA that produced the same IF pattern. Pattern 3 was observed in sera that contained antinuclear antibodies as detected by IF on HEp-2 cells.

Duke Scholars

Published In

J Rheumatol

ISSN

0315-162X

Publication Date

April 1991

Volume

18

Issue

4

Start / End Page

575 / 579

Location

Canada

Related Subject Headings

  • Peroxidase
  • Neutrophils
  • Humans
  • Granulocytes
  • Fluorescent Antibody Technique
  • Enzyme-Linked Immunosorbent Assay
  • Cell Line
  • Arthritis & Rheumatology
  • Antibodies, Antinuclear
  • Antibodies
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Christenson, V. D., Dooley, M. A., & Allen, N. B. (1991). Discrimination of antineutrophil antibodies from antinuclear antibodies using immunofluorescence on neutrophils and HL60 cells. J Rheumatol, 18(4), 575–579.
Christenson, V. D., M. A. Dooley, and N. B. Allen. “Discrimination of antineutrophil antibodies from antinuclear antibodies using immunofluorescence on neutrophils and HL60 cells.J Rheumatol 18, no. 4 (April 1991): 575–79.
Christenson, V. D., et al. “Discrimination of antineutrophil antibodies from antinuclear antibodies using immunofluorescence on neutrophils and HL60 cells.J Rheumatol, vol. 18, no. 4, Apr. 1991, pp. 575–79.

Published In

J Rheumatol

ISSN

0315-162X

Publication Date

April 1991

Volume

18

Issue

4

Start / End Page

575 / 579

Location

Canada

Related Subject Headings

  • Peroxidase
  • Neutrophils
  • Humans
  • Granulocytes
  • Fluorescent Antibody Technique
  • Enzyme-Linked Immunosorbent Assay
  • Cell Line
  • Arthritis & Rheumatology
  • Antibodies, Antinuclear
  • Antibodies