Skip to main content
Journal cover image

Rabbits immunized with a peptide encoded for by the 230-kD bullous pemphigoid antigen cDNA develop an enhanced inflammatory response to UVB irradiation: a potential animal model for bullous pemphigoid.

Publication ,  Journal Article
Hall, RP; Murray, JC; McCord, MM; Rico, MJ; Streilein, RD
Published in: J Invest Dermatol
July 1993

Previous attempts to develop an animal model of bullous pemphigoid (BP) have failed to result in inflammatory disease in the skin. P1-2 is an 18-amino acid peptide encoded for by the 230-kD BP antigen cDNA that has been shown to contain an epitope recognized by circulating antibodies from patients with BP. The purpose of this study was to determine if ultraviolet B irradiation of rabbits after immunization with the P1-2 peptide would result in an enhanced inflammatory response in the skin to that injury. Three rabbits were immunized with either P1-2 or a control peptide. All rabbits immunized with P1-2, and none of the control rabbits, developed antibodies against P1-2 that bound in vitro to both human and rabbit skin in a linear pattern at the basement membrane zone. Immunized rabbits were irradiated on the flank with ultraviolet light. Rabbits immunized with P1-2 developed an enhanced inflammatory reaction to ultraviolet B irradiation leading to epidermal necrosis and sloughing of some sites in 6-9 d. Control rabbits showed only mild erythema without sloughing, which healed in 4-6 d. Histology in the P1-2 immunized rabbits at 24 h revealed an inflammatory infiltrate of neutrophils at the dermal-epidermal junction, whereas control rabbits showed only mild edema and a sparse inflammatory infiltrate. All the rabbits immunized with P1-2 had linear deposits of immunoglobulin G and C3 at the basement membrane zone of healed skin compared to none of the controls. These findings demonstrate that antibodies against a synthetic peptide encoded by the BP antigen 1 sequence can lead to an enhanced inflammatory response after epithelial injury in rabbit skin.

Duke Scholars

Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

July 1993

Volume

101

Issue

1

Start / End Page

9 / 14

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Skin
  • Radiation Injuries, Experimental
  • Rabbits
  • Peptides
  • Pemphigoid, Bullous
  • Non-Fibrillar Collagens
  • Nerve Tissue Proteins
  • Immunization
  • Fluorescent Antibody Technique
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hall, R. P., Murray, J. C., McCord, M. M., Rico, M. J., & Streilein, R. D. (1993). Rabbits immunized with a peptide encoded for by the 230-kD bullous pemphigoid antigen cDNA develop an enhanced inflammatory response to UVB irradiation: a potential animal model for bullous pemphigoid. J Invest Dermatol, 101(1), 9–14. https://doi.org/10.1111/1523-1747.ep12358276
Hall, R. P., J. C. Murray, M. M. McCord, M. J. Rico, and R. D. Streilein. “Rabbits immunized with a peptide encoded for by the 230-kD bullous pemphigoid antigen cDNA develop an enhanced inflammatory response to UVB irradiation: a potential animal model for bullous pemphigoid.J Invest Dermatol 101, no. 1 (July 1993): 9–14. https://doi.org/10.1111/1523-1747.ep12358276.
Journal cover image

Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

July 1993

Volume

101

Issue

1

Start / End Page

9 / 14

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Skin
  • Radiation Injuries, Experimental
  • Rabbits
  • Peptides
  • Pemphigoid, Bullous
  • Non-Fibrillar Collagens
  • Nerve Tissue Proteins
  • Immunization
  • Fluorescent Antibody Technique